1.10630

AFB-Color malachite green (oxalate) solution

for the microscopic inverstigation of acid-fast bacteria (cold staining)

• 分類程式碼代碼: 41116130
• NACRES: NA.74

属性

• 品質等級: 400
• 形狀: liquid
• IVD: for in vitro diagnostic use
• 密度: 1.0 g/cm³ at 20 °C
• 應用: clinical testing; diagnostic assay manufacturing; hematology; histology
• 儲存溫度: 15-25°C

说明

一般說明

AFB-Color malachite green (oxalate) solution - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining), is a ready-to-use solution used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin., for example smears of enriched bacterial cultures. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. In this case, a modification of the AFB-Color carbol fuchsin (Product number 1.08512) solution renders heating unnecessary (Kinyoun method, cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol^™ (Product number 1.08000) dissolves the bacteria from the surrounding viscid sputum and cell material.
The 500 ml bottle of AFB-Color malachite green (oxalate) solution provides 250 stainings. It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

應用

Used to detect mycobacteria (AFB cold staining)

分析報告

Suitability for microscopypasses testMycobacteriaredBackgroundlight green

法律資訊

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable