07-1477
Anti-Rsk1 Antibody
from rabbit, purified by affinity chromatography
别名:
90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase alpha-1, S6K-alpha 1, p90-RSK 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase, 90kD, 1, Ribosmal protein S
登录查看公司和协议定价
所有图片(2)
About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推荐产品
生物源
rabbit
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
rat, mouse, human
技術
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... RPS6KA1(6195)
一般說明
Rsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c. Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression. Rsk1 is able to phosphorylate Ser133 of CREB, activating it as a transcription factor. Rsk1 activation can be monitored by phosphorylation state specific antibodies directed against sites involved in its activation.
特異性
Recognizes p90 RSK1.
免疫原
Synthetic peptide corresponding to the c-terminal sequence of human p90 RSK1 (C-LAQRRVRKLPSTTL).
應用
Anti-Rsk1 Antibody is an antibody against Rsk1 for use in WB, IP, ELISA.
Immunoprecipitation: 4 µg of a previous lot of this antibody was used per 1500 µg of crude protein sample in Immunoprecipitation.
ELISA: Recommended.
Optimal working dilutions must be determined by end user.
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
ELISA: Recommended.
Optimal working dilutions must be determined by end user.
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
品質
Routinely evaluated by Western Blot on NIH/3T3 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution of this lot detected RSK1 on 10 μg of NIH/3T3 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution of this lot detected RSK1 on 10 μg of NIH/3T3 lysates.
標靶描述
83 kDa
聯結
Replaces: 04-417
外觀
Purified rabbit polyclonal IgG in buffer containing TBS with 0.01% Sodium Azide in 50% glycerol.
儲存和穩定性
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Not finding the right product?
Try our 产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
Pricing of drugs and donations: options for sustainable equity pricing.
C Perez-Casas,E Herranz,N Ford
Tropical Medicine & International Health null
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门