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A9857

Sigma-Aldrich

αα-Amilasa from Aspergillus oryzae

greener alternative

≥150 units/mg protein (biuret)

Sinónimos:

1,4-α-D-glucano glucanohidrolasa

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About This Item

Comisión internacional de enzimas:
3.2.1.1 (BRENDA, IUBMB)
Número CE:
232-588-1
Número MDL:
MFCD00081319
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Formulario

powder

Nivel de calidad

200

actividad específica

≥150 units/mg protein (biuret)

características de los productos alternativos más sostenibles

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

categoría alternativa más sostenible

Enabling,

temp. de almacenamiento

−20°C

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Descripción general

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Aplicación

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis [1]. Amylases from Aspergillus oryzae are commonly used as baking additives to prevent staling in the baking industry, clarify haze from fruit juices and alcoholic beverages, and to produce glucose and maltose syrup products [2].

Acciones bioquímicas o fisiológicas

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. The International Union of Immunological Societies (IUIS) has classified α-amylase from Aspergillus oryzae (Asp o II) as an occupational allergen that can cause respiratory symptoms [2].

Calidad

Crude

Definición de unidad

One unit will liberate 1.0mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Forma física

powder

Otras notas

Véase más información sobre enzimas para análisis de carbohidratos complejos en www.sigma-aldrich.com/enzymeexplorer

sustrato

Referencia del producto
Descripción
Precios

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H302,H334

Clasificaciones de peligro

Acute Tox. 4 Oral - Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000405206
0000396724
0000396725
0000396726
0000387746

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X Baur et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 24(5), 465-470 (1994-05-01)
The commercially available alpha-amylase from Aspergillus oryzae which is widely used as a baking additive was compared with a highly purified enzyme preparation. We used enzyme allergosorbent test (EAST), EAST inhibition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focussing, immunoblotting
Marit Navis et al.
Nutrients, 12(11) (2020-11-08)
Human milk is the optimal diet for infant development, but infant milk formula (IMF) must be available as an alternative. To develop high-quality IMF, bovine milk processing is required to ensure microbial safety and to obtain a protein composition that
Sean McClure
Journal of AOAC International, 103(4), 1060-1072 (2020-11-27)
A multi-laboratory study was completed for AOAC First Action Method 2015.14. Ten laboratories from eight countries participated. Each laboratory analyzed (in duplicate) a placebo and 14 fortified nutritionals. Product matrices analyzed included milk, soy, partially hydrolyzed milk, partially hydrolyzed soy
Jesus Valcarcel et al.
Polymers, 14(1) (2022-01-12)
Styela clava is an edible sea squirt farmed in Korea that has gradually invaded other seas, negatively impacting the ecology and economy of coastal areas. Extracts from S. clava have shown wide bioactivities, and ascidians have the unique capability among
Fengyuan Liu et al.
Food & function, 13(6), 3220-3233 (2022-02-26)
Both the liberation and stability of endogenous folate are relevant to the bioaccessibility of folate. Since folates are unstable, in addition to studying the natural folate content in foods, bioaccessibility should be considered. To understand folate changes during digestion, a
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Protocolos

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Questions

  1. Please specify quantity for 250KU product no A9857

    1 answer

    1. This product is sold by activity. The minimum activity specification for this material is 150 units per milligram. The weight of this product can be determined using the activity value reported on the lot-specific Certificate of Analysis(COA). For example, the sample COA has an activity value of 280 units/mg. The amount of powder in the 250,000-unit package will be 892.85mg.

      Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/a9857#product-documentation

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