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Key Documents

SAB4301138

Sigma-Aldrich

Anti-GFP antibody produced in rabbit

affinity isolated antibody

Synonyme(s) :

GFP-like chromoprotein

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

27 kDa

Concentration

3.2 mg/mL

Technique(s)

western blot: 1:1000-1:10000 (Cell Lysate)

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Green fluorescent protein (GFP) is a 27kDa protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+- activated photoprotein aequorin to GFP.

Spécificité

The antibody detects transfected proteins containing GFP tag.

Immunogène

Full length fusion protein

Application

Anti-GFP antibody produced in rabbit has been used in:
  • double immunofluorescence staining
  • immunoblot analysis.
  • immunoprecipitation.

Actions biochimiques/physiologiques

Green fluorescent protein (GFP) is a reporter molecule which is used for checking gene expression and protein localization in vivo, in situ and in real time. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells. GFP is considered as a unique tool to monitor dynamic processes in several living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

UGT79B31 is responsible for the final modification step of pollen-specific flavonoid biosynthesis in Petunia hybrida.
Knoch E, et al.
Planta, 247(4), 779-790 (2018)
Minna M Koskela et al.
The Plant cell, 30(8), 1695-1709 (2018-07-04)
The amount of light energy received by the photosynthetic reaction centers photosystem II (PSII) and photosystem I (PSI) is balanced through state transitions. Reversible phosphorylation of a light-harvesting antenna trimer (L-LHCII) orchestrates the association between L-LHCII and the photosystems, thus
David A Rhodes et al.
Frontiers in immunology, 9, 662-662 (2018-04-20)
Activation of human Vγ9/Vδ2 T cells by "phosphoantigens" (pAg), the microbial metabolite (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP) and the endogenous isoprenoid intermediate isopentenyl pyrophosphate, requires expression of butyrophilin BTN3A molecules by presenting cells. However, the precise mechanism of activation of Vγ9/Vδ2 T
Green fluorescent protein as a reporter for macromolecular localization in bacterial cells.
Margolin W
Methods, 20(1), 62-72 (2000)
RNA-dependent RNA polymerases of both virulent and benign rabbit caliciviruses induce striking rearrangement of Golgi membranes.
Urakova N, et al.
PLoS ONE, 12(1), e0169913-e0169913 (2017)

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