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KCQS01

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix™

Low ROX™, for ABI and Stratagene instruments

Synonyme(s) :

qPCR master mix, sybr green qPCR

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Forme

liquid

Utilisation

sufficient for 1250 reactions
 sufficient for 250 reactions
 sufficient for 5000 reactions

Caractéristiques

dNTPs included
hotstart

Conditions de stockage

protect from light

Technique(s)

qPCR: suitable

Couleur

colorless

Entrée

purified DNA

Compatibilité

for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI ViiA 7
for use with Agilent AriaMx
for use with Douglas Scientific IntelliQube
for use with Qiagen Rotor-Gene Q
for use with QuantStudio™
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

Méthode de détection

SYBR® Green

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Application

KiCqStart® SYBR® Green qPCR ReadyMix™ has been used:
  • for the amplification and quantification of DNA in real-time PCR (qPCR) assay
  • in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR)
  • in the amplification of complementary DNA (cDNA) by real-time PCR (qPCR) assay
PCR applications:
  • Gene expression
  • DNA quantification
  • CHiP

Caractéristiques et avantages

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Composants

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Qualité

Kit components are free of contaminating DNase and RNase. KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (r2 > 0.995) and a PCR efficiency > 90%.

Autres remarques

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Informations légales

6538 is a trademark of American Type Culture Collection
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
Mx3000P is a registered trademark of Agilent Technologies, Inc.
Mx3005P is a registered trademark of Agilent Technologies, Inc.
Mx4000 is a trademark of Agilent Technologies, Inc.
QuantStudio is a trademark of IROA Technologies LLC
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
SYBR is a registered trademark of Life Technologies

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Plasmodium falciparum EPCR-binding PfEMP1 expression increases with malaria disease severity and is elevated in retinopathy negative cerebral malaria
<BIG><BIG>Shabani E, et al.</BIG></BIG>
BMC Medicine, 15, 183-183 (2017)
Engineered zinc-finger transcription factors activate OCT4 (POU5F1), SOX2, KLF4, c-MYC (MYC) and miR302/367
<BIG><BIG>Ji Q, et al.</BIG></BIG>
Nucleic Acids Research, 42, 6158-6167 (2014)
Estela Shabani et al.
BMC medicine, 15(1), 183-183 (2017-10-14)
Expression of group A and the A-like subset of group B Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is associated with severe malaria (SM). The diversity of var sequences combined with the challenges of distinct classification of patient pathologies has
Jemere Bekele Harito et al.
Water research, 114, 228-236 (2017-03-02)
Although standard methods for analyzing water samples for the protozoan parasites Cryptosporidium spp. and Giardia duodenalis are available and widely used, equivalent methods for analyzing water samples for Toxoplasma gondii oocysts are lacking. This is partly due to the lack
Jemere Bekele Harito et al.
Water research, 127, 68-76 (2017-10-17)
Proof-of-principle of lectin-magnetic separation (LMS) for isolating Toxoplasma oocysts (pre-treated with 0.5% acidified pepsin (AP)) from water for subsequent detection by microscopy or molecular methods has been shown. However, application of this technique in the routine water-analysis laboratory requires that
Afficher tous les articles scientifiques apparentés

Articles

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

Analyse des données de PCR/qPCR

Après une PCR classique, l'analyse des données de PCR/qPCR est effectuée par une séparation sur un gel d'agarose, ou depuis peu, à travers un capillaire.

Analyse des données de PCR/qPCR

Après une PCR classique, l'analyse des données de PCR/qPCR est effectuée par une séparation sur un gel d'agarose, ou depuis peu, à travers un capillaire.

Analyse des données de PCR/qPCR

Après une PCR classique, l'analyse des données de PCR/qPCR est effectuée par une séparation sur un gel d'agarose, ou depuis peu, à travers un capillaire.

Afficher tout

Protocoles

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Contenu apparenté

SYBR® Green Based Quantitative PCR

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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