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KCQS03

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix

iQ, with fluorescein for Bio-Rad systems

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Forme

liquid

Utilisation

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

Caractéristiques

dNTPs included
hotstart

Conditions de stockage

protect from light

Technique(s)

qPCR: suitable

Couleur

colorless

Entrée

purified DNA

Compatibilité

for use with Bio-Rad MyiQ
for use with Bio-Rad iCycler iQ
for use with Bio-Rad iQ 5

Méthode de détection

SYBR® Green

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Application

KiCqStart® SYBR® Green qPCR ReadyMix has been used for the qPCR analysis of 16S rRNA.
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
PCR applications:
  • Gene expression
  • DNA quantification
  • CHiP

Caractéristiques et avantages

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Composants

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Autres remarques

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Informations légales

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iQ is a trademark of Bio-Rad Laboratories, Inc.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Himanshu Kumar Khuntia et al.
SN applied sciences, 2(8), 1320-1320 (2020-08-25)
This research aims to determine the presence of antibiotic-resistant genes (ARG) in anaerobic biofilm reactors (ABR) fed with household chemical products (HCP) such as laundry detergents and handwash without any influx of antibiotics. The ABR comprised a three-chamber design with
Jackson T Sparks et al.
Insect biochemistry and molecular biology, 43(12), 1161-1171 (2013-10-26)
The yellow-fever mosquito, Aedes aegypti, infects a growing number of people every year with dengue, yellow fever and chikungunya viruses. Contact chemoreception in mosquitoes influences a number of behaviors including host-selection, oviposition and feeding. While these behaviors are in many
Glade Dlott et al.
Journal of microbiological methods, 115, 112-120 (2015-06-10)
We tested a method of estimating the activity of detectable individual bacterial and archaeal OTUs within a community by calculating ratios of absolute 16S rRNA to rDNA copy numbers. We investigated phylogenetically coherent patterns of activity among soil prokaryotes in
Christine E Prasse et al.
Applied and environmental microbiology, 81(10), 3482-3491 (2015-03-15)
Restored wetland soils differ significantly in physical and chemical properties from their natural counterparts even when plant community compositions are similar, but effects of restoration on microbial community composition and function are not well understood. Here, we investigate plant-microbe relationships
Tyler J Kirby et al.
Journal of applied physiology (Bethesda, Md. : 1985), 119(4), 321-327 (2015-06-07)
The ability of skeletal muscle to hypertrophy in response to a growth stimulus is known to be compromised in older individuals. We hypothesized that a change in the expression of protein-encoding genes in response to a hypertrophic stimulus contributes to

Articles

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

Après une PCR classique, l'analyse des données de PCR/qPCR est effectuée par une séparation sur un gel d'agarose, ou depuis peu, à travers un capillaire.

Après une PCR classique, l'analyse des données de PCR/qPCR est effectuée par une séparation sur un gel d'agarose, ou depuis peu, à travers un capillaire.

Après une PCR classique, l'analyse des données de PCR/qPCR est effectuée par une séparation sur un gel d'agarose, ou depuis peu, à travers un capillaire.

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Protocoles

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Contenu apparenté

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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