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L6544

Sigma-Aldrich

LuminoCt® SYBR® Green qPCR ReadyMix™

For fast SYBR Green quantitative PCR

Synonyme(s) :

LuminoCt®

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Niveau de qualité

200

Forme

liquid

Utilisation

sufficient for 100 reactions
 sufficient for 2000 reactions
 sufficient for 500 reactions

Caractéristiques

dNTPs included
hotstart

Technique(s)

qPCR: suitable

Couleur

colorless

Entrée

purified DNA

Application(s)

agriculture

Compatibilité

Bio-Rad MyiQ
 Bio-Rad iCycler iQ
 Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

Méthode de détection

SYBR® Green

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Description générale

LuminoCt® SYBR® Green qPCR ReadyMix is ready to use master mix that combines the performance enhancements of our JumpStart™ Taq antibody for hot start. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.
  • Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
  • ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
  • Kit is not compatible with qPCR instruments that utilize glass capillary tubes

Application

LuminoCt® SYBR® Green qPCR ReadyMix™ has been used for fast quantitative polymerase chain reaction (qPCR) and quantitative RT-PCR amplifications. It has also been used in qPCR for measuring gene expression.

Caractéristiques et avantages

  • Ideal for performing high-throughput, quantitative PCR applications
  • The master mix allows consistency from one reaction to the next
  • Reduced preparation time and reduced risk of contamination from multiple pipetting steps
  • The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • LuminoCt® ReadyMixes require the use of small amplicons (<200bp) for optimal results
  • This master mix is compatible with commercial primer sets, including TaqMan® assays
  • Assay results in as little as 25 minutes
  • Unsurpassed accuracy, precision, and sensitivity
  • Virtually eliminates the need to optimize assay parameters

Conditionnement

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Autres remarques

  • At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
  • Sigma recommends designing primers and probes with Beacon Designer software
  • To order primers and probes from Sigma, visit: www.sigma.com/oligos

Informations légales

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LuminoCt is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
SabreLite is a registered trademark of Pelican Products, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Composants de kit seuls

Réf. du produit
Description
  • 2 X

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • R4526100X ROX internal reference dyeFDS

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

S4438

JumpStart™ Taq ReadyMix avec SYBR® Green™, for quantitative PCR, MgCI2 in buffer

S1816

SYBR® Green JumpStart™ Taq ReadyMix™ for Quantitative PCR, Capillary Formulation, SYBR® Green qPCR reagent for Roche LightCycler® capillary systems

S5193

JumpStart™ Taq ReadyMix avec SYBR® Green™, for quantitative PCR, without MgCl2 in buffer

S9194

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR, SYBR® Green qPCR reagent, passive reference dye included

KCQS00

KiCqStart® SYBR® Green qPCR ReadyMix™, For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

KCQS01

KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX™, for ABI and Stratagene instruments

KCQS02

KiCqStart® SYBR® Green qPCR ReadyMix™, with ROX™ for ABI instruments

KCQS03

KiCqStart® SYBR® Green qPCR ReadyMix™, iQ™, with fluorescein for Bio-Rad systems

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Jan Gravemeyer et al.
Oncogene, 41(1), 37-45 (2021-10-21)
Merkel cell carcinoma (MCC) is a neuroendocrine tumor either induced by integration of the Merkel cell polyomavirus into the cell genome or by accumulation of UV-light-associated mutations (VP-MCC and UV-MCC). Whether VP- and UV-MCC have the same or different cellular
Khrystyna Levytska et al.
PPAR research, 2014, 637251-637251 (2014-04-09)
Common pregnancy complications, such as severe preeclampsia and intrauterine growth restriction, disrupt pregnancy progression and impair maternal and fetal wellbeing. Placentas from such pregnancies exhibit lesions principally within the syncytiotrophoblast (SCT), a layer in direct contact with maternal blood. In
Oksana Shynlova et al.
Journal of cellular and molecular medicine, 17(2), 311-324 (2013-02-06)
Leucocyte infiltration in the decidua (maternal-foetal interface) before, during and after term (TL) and preterm labour (PTL) was studied in mouse. We also investigated the mechanism of peripheral leucocyte recruitment into decidua by analysing the tissue cytokine profiles. Decidual tissues
Oksana Shynlova et al.
Journal of cellular and molecular medicine, 17(1), 90-102 (2012-12-05)
This study aimed to determine the mechanism of uterine activation during labour, both term (TL) and preterm (PTL). We hypothesized that the peripheral leucocytes are recruited to uterine tissues by locally produced cytokines where they contribute to the initiation of
Devika Sharanya et al.
Evolution & development, 17(1), 34-48 (2015-01-30)
Studies of vulval development in the nematode C. elegans have identified many genes that are involved in cell division and differentiation processes. Some of these encode components of conserved signal transduction pathways mediated by EGF, Notch, and Wnt. To understand
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PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

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PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

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PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

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Protocoles

Protocole de qPCR universel avec SYBR Green

Notre protocole de qPCR avec SYBR Green est conçu pour quantifier précisément l'expression génique ou des réactions de RT-PCR.

Contenu apparenté

SYBR® Green Based Quantitative PCR

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

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