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SAB4200559

Sigma-Aldrich

Anti-VAChT (C-terminal) antibody produced in rabbit

IgG fraction of antiserum

Synonyme(s) :

Anti-SLC18A3, Anti-Solute carrier family 18 member 3, Anti-VAT, Anti-vesicular acetylcholine transporter

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

rat, mouse, human

Technique(s)

indirect immunofluorescence: 1:1,000-1:2,000 using PC12 cells treated with rat NGF.
western blot: 1:500-1:800 using extracts of mouse brain (S1 fraction).

Numéro d'accès UniProt

Q16572

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... SLC18A3(6572)
mouse ... slc18a3(20508)
rat ... slc18a3(60422)

Description générale

Vesicular Acetylcholine Transporter (VAChT, SLC18A3) belongs to the solute carrier family 18 (SLC18) family of proton/neurotransmitter antiporters and shares a high degree of homology to vesicular monoamine transporters (VMATs). It is a 12-transmembrane domain protein. VAChT is expressed in all major cholinergic neurons in the central and peripheral nervous system and is mainly localized in small synaptic vesicles in cholinergic nerve terminals.

Immunogène

synthetic peptide corresponding to a sequence at the C-terminus of human vesicular acetylcholine transporter (VAChT), conjugated to KLH. The corresponding sequence is highly conserved in rat and mouse VAChT (82% identity).

Application

Anti-VAChT (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence staining
  • immunocytochemistry
  • double immunolabeling

Actions biochimiques/physiologiques

Vesicular Acetylcholine Transporter (VAChT), is required in cholinergic neurons for the selective transport of acetylcholine (ACh) into synaptic vesicles. It depends on the activity of a vacuolar-type H+ -ATPase to accumulate ACh in synaptic vesicles. Knockdown of VAChT in the hippocampus has been shown to cause behavior changes, learning deficits, specific deficits in spatial memory and long-term potentiation (LTP). VAChT provides a specific marker for cholinergic neurons, for the study of cholinergic transmission in experimental models of Alzheimer′s disease and other nervous system disorders. Cloning of the VAChT gene has revealed a single genetic locus encoding both VAChT and ChAT, providing a unique genomic arrangement suggesting that these genes may be co-regulated.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Vesicular acetylcholine transporter defect underlies devastating congenital myasthenia syndrome
Aran A, et al.
Neurology, 88(11), 1021-1028 (2017)
Long Li et al.
Frontiers in aging neuroscience, 13, 770948-770948 (2022-02-22)
To explore the effect of electro-acupuncture (EA) treatment on pattern separation and investigate the neural circuit mechanism involved in five familial mutations (5 × FAD) mice. Five familial mutations mice were treated with EA at Baihui (DU20) and Shenting (DU24)
Alzheimer's disease: targeting the cholinergic system
Ferreira-Vieira TH, et al.
Current Neuropharmacology, 14(1), 101-115 (2016)
Elimination of the vesicular acetylcholine transporter in the forebrain causes hyperactivity and deficits in spatial memory and long-term potentiation
Martyn AC, et al.
Proceedings of the National Academy of Sciences of the USA, 109(43), 17651-17656 (2012)
Weilin Liu et al.
Alzheimer's research & therapy, 14(1), 53-53 (2022-04-15)
The degeneration of the cholinergic circuit from the basal forebrain to the hippocampus contributes to memory loss in patients suffering from Alzheimer's disease (AD). However, the internal relationships between the acetylcholine (Ach) cycle and memory decline during the early stages
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