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A5795

Sigma-Aldrich

Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

HRP Rabbit Anti-Rat IgG (whole molecule)

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About This Item

Numéro MDL:
MFCD00162813
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

200

Conjugué

peroxidase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

rat

Ne doit pas réagir avec

human

Technique(s)

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:750
western blot (chemiluminescent): 1:80,000-1:160,000 using rat PC12 cell extract

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat Igs and does not react with human IgG.

Immunogène

purified rat IgG

Application

Anti-Rat IgG (whole molecule)-Peroxidase antibody has been used as a secondary antibody in immunohistochemical applications at a dilution of 1:1500. The product has also been used for western blot analysis at 1:20,000. Furthermore, the antibody can be used for direct ELISA at 1:50,000.
Anti-Rat IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in immunohistochemistry and western blot analysis.
HA-tagged proteins over expressed in S. cerevisiae were detected by western blot using HRP-conjugated rabbit anti-rat IgG as the secondary antibody.

Actions biochimiques/physiologiques

Immunoglobulin (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgG participates in hypersensitivity type II and type III.

Forme physique

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Notes préparatoires

Adsorbed with human IgG to reduce background staining with human samples.
Prepared by two-step glutaraldehyde method.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Medhat A Al-Ghobashy et al.
Scientific reports, 7, 46468-46468 (2017-04-21)
Recombinant human myelin basic protein (rhMBP) was previously produced in the milk of transgenic cows. Differences in molecular recognition of either hMBP or rhMBP by surface-immobilized anti-hMBP antibodies were demonstrated. This indicated differences in immunological response between rhMBP and hMBP.
Probing the Function of STAS Domains of the Arabidopsis Sulfate Transporters.
Shibagaki, N.
Journal of Biochemistry, 279, 30791-30799 (2004)
Galectin-3 as a potential therapeutic target in tumors arising from malignant endothelia
Johnson KD, et al.
Neoplasia, 9(8), 662-662 (2007)
Sandra Bensmihen et al.
FEBS letters, 561(1-3), 127-131 (2004-03-12)
The Arabidopsis abscisic acid (ABA) insensitive (ABI)5 transcription factor participates in the ABA-dependent induction of late embryogenesis abundant (LEA) genes in the final stages of seed development. We tested whether the VP16 transcriptional activation domain is sufficient to provide ABI5
Kim D Johnson et al.
Neoplasia (New York, N.Y.), 9(8), 662-670 (2007-09-06)
Angiosarcoma (ASA) in humans and hemangiosarcoma (HSA) in dogs are deadly neoplastic diseases characterized by an aggressive growth of malignant cells with endothelial phenotype, widespread metastasis, and poor response to chemotherapy. Galectin-3 (Gal-3), a beta-galactoside-binding lectin implicated in tumor progression
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