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Key Documents

A9042

Sigma-Aldrich

Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Rabbit Anti-Dog IgG (whole molecule)−HRP

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

Conjugué

peroxidase conjugate

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:25,000
dot blot: 1:40,000 using direct chemiluminescence assay using 10 ng dog IgG/dot. Luminol plus enhancer was used as substrate.

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Immunoglobulins (Igs) belong to immunoglobulin super-family. IgG is abundant immunoglobulin in proteins in human serum. The four classes of IgG can be IgG1, IgG2, IgG3 and IgG4.

Spécificité

Anti-dog IgG antibody is specific for IgG in dogs.

Immunogène

Dog IgG

Application

Anti-Dog IgG (whole molecule)-peroxidase antibody has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • IgG/dot
  • western blotting

Actions biochimiques/physiologiques

Dog IgG is a glycoprotein antibody that mediates intestinal antibacterial defense mechanisms. It may regulate the phagocytosis of Gram-negative bacteria . Anti-dog IgG antibody is specific for IgG in dogs.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Notes préparatoires

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Characterization of the Major Antigenic Protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and Its Application for Serodiagnosis of Ehrlichiosis.
Knowles, T., et al.
Clinical and Vaccine Immunology : CVI, 10(4), 520-524 (2003)
Xiaohui Yao et al.
Frontiers in cellular and infection microbiology, 13, 1291937-1291937 (2024-01-18)
The Hedi virus (HEDV) and Wuxiang virus (WUXV) are newly discovered Bunyaviruses transmitted by sandflies. The geographical distribution of isolation of these two viruses continues to expand and it has been reported that WUXV causes neurological symptoms and even death
R J Heddle et al.
Immunology, 29(1), 197-208 (1975-07-01)
Secretory IgA antibodies to Vibrio cholerae were purified from the parotid saliva and mammary secretions of locally and orally immunized dogs using gel filtration, ion-exchange chromatography and anti-immunoglobulin immuno-absorbents. IgM and IgG antibodies were isolated from serum by gel filtration
Maria Agallou et al.
PloS one, 11(2), e0149894-e0149894 (2016-02-26)
Leishmania infantum is the etiologic agent of zoonotic visceral leishmaniasis (VL) in countries in the Mediterranean basin, where dogs are the domestic reservoirs and represent important elements in the transmission of the disease. Since the major focal areas of human
IgG subclasses and allotypes: from structure to effector functions
Vidarsson G, et al.
Frontiers in Immunology, 5(2), 520-520 (2014)

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