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12013819001

Roche

Anti-HA-Peroxidase, High Affinity

from rat IgG1

Synonyme(s) :

antibody

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About This Item

Code UNSPSC :
12352203

Source biologique

rat

Niveau de qualité

Conjugué

peroxidase conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

clone 3F10, monoclonal

Forme

lyophilized (clear, colorless solution after reconstitution)

Conditionnement

pkg of 25 U (25 μg)

Fabricant/nom de marque

Roche

Isotype

IgG1

Séquence de l'épitope

YPYDVPDYA

Température de stockage

2-8°C

Catégories apparentées

Description générale

Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.

Spécificité

Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogène

The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.

Application

  • Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA
  • Western blot
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

Notes préparatoires

Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • Dot blot: 50 mU/ml
  • ELISA: 25 mU/ml
  • Western blot: 50 mU/ml

Reconstitution

Add 1.0 ml double-distilled water for a final concentration of 25 U/mL.
Rehydrate for 10 min prior to use.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

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Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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Cancers, 12(6) (2020-06-25)
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Julia K Günther et al.
Biology, 9(5) (2020-05-21)
Rigosertib, via reactive oxygen species (ROS), stimulates cJun N-terminal kinases 1/2 (JNK1/2), which inactivate RAS/RAF signaling and thereby inhibit growth and survival of tumor cells. JNK1/2 are not only regulated by ROS-they in turn can also control ROS production. The
Satoshi Hashimoto et al.
Scientific reports, 10(1), 3422-3422 (2020-02-27)
Ribosome stalling triggers the ribosome-associated quality control (RQC) pathway, which targets collided ribosomes and leads to subunit dissociation, followed by proteasomal degradation of the nascent peptide. In yeast, RQC is triggered by Hel2-dependent ubiquitination of uS10, followed by subunit dissociation
Jihoon Ha et al.
International journal of molecular sciences, 21(9) (2020-05-02)
p62/sequestosome-1 is a scaffolding protein involved in diverse cellular processes such as autophagy, oxidative stress, cell survival and death. It has been identified to interact with atypical protein kinase Cs (aPKCs), linking these kinases to NF-κB activation by tumor necrosis

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