A6792
Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Rabbit Anti-Dog IgG (whole molecule)−HRP
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
peroxidase conjugate
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Technique(s)
direct ELISA: 1:10,000
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
IgG (immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mammalian IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
Specificity of the anti-dog IgG antiserum is determined by immunoelectrophoresis, prior to conjugation, versus normal dog serum and dog IgG.
Application
Anti-Dog IgG (whole molecule) Peroxidase antibody produced in rabbit has been used:
- for the detection of antibodies against noro viruses, by ELISA (enzyme linked immunosorbent asay) at 1:2000 for 1 hour at 37°C
- in IgG (Immunoglobulin G) staining method
- in ELISA to detect the incorporation of RVGTM (a rabies virus G protein variant)
Actions biochimiques/physiologiques
IgG (Immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. Anti-Dog IgG antibody is a secondary antibody. Second antibodies (secondary antibodies) are raised against primary antibodies, which bind to specific antigens to create antigen antibody complexes.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.05% MIT
Notes préparatoires
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Informations légales
Ampliflu is a trademark of Sigma-Aldrich Co. LLC
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Les clients ont également consulté
Juliano Bordignon et al.
Revista do Instituto de Medicina Tropical de Sao Paulo, 44(3), 151-154 (2002-08-07)
The determination of the rabies neutralizing antibody (VNA) response after immunization against rabies is an acceptable index of the efficacy of a vaccine and a successful treatment. Several tests have been developed in attempt to improve the assessment of VNA
Immunohistochemical detection of IgM and IgG in lung tissue of dogs with leptospiral pulmonary haemorrhage syndrome (LPHS)
Schuller S, et al.
Comparative Immunology, Microbiology and Infectious Diseases, 40, 47-53 (2015)
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Control of canine visceral leishmaniasis (CVL), a major zoonotic disease in Brazil and many other tropical and subtropical countries, remains difficult as an accurate and reliable diagnosis is still missing. In endemic regions, infected dogs are the main parasitic reservoir
Morten Tryland et al.
Journal of wildlife diseases, 48(3), 632-645 (2012-06-29)
Information on health parameters, such as antibody prevalences and serum chemistry that can reveal exposure to pathogens, disease, and abnormal physiologic conditions, is scarce for Antarctic seal species. Serum samples from Antarctic fur seals (Arctocephalus gazella, n=88) from Bouvetøya (2000-2001
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