S6436
Anti-phospho-S6-Kinase (pThr421/pSer424) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Synonyme(s) :
Anti-phospho-p70S6K (pThr421/pSer424)
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
buffered aqueous glycerol solution
Espèces réactives
mouse, rat, human
Technique(s)
immunoprecipitation (IP): 1:250
western blot (chemiluminescent): 1:1,000
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... RPS6KB1(6198)
mouse ... Rps6kb1(72508)
rat ... Rps6kb1(83840)
Catégories apparentées
Description générale
S6 kinase (p70s6k) is activated by an upstream kinase, mTOR in response to nutrients and growth factors. S6 kinase (p70s6k) is a critical regulatory component and mTOR along with the eukaryotic initiation factor 4E (eIF4E)–binding protein 1 (4E-BP1) and S6 kinase (p70s6k) leads to increased ribosomal biogenesis and hence translation. As a critical component and has the ability to modify the cellular responses to availability of nutrients, and growth factors like insulin and IGF-1 when mTOR is chronically active. In presence of excess and chronic insulin, or high fat diets, S6 kinase (p70s6k) regulates by a negative feedback loop and constitutively shuts down the responsiveness of cells to insulin. Thus, mTOR through the S6 kinase (p70s6k) plays a pivotal role in the development of insulin resistance and type 2 diabetes. mTOR signaling via p70S6 kinase also drives cell growth and proliferation, cancer and aging.
Anti-phospho-S6-kinase (p70S6K) (phosphothreonine 421/phosphoserine424) detects p70 S6 kinase and p85 S6 kinase only when activated by phosphorylation at Thr421/Ser424 and does not cross-react with other phosphoylated protein kinases.
Anti-phospho-S6-kinase (p70S6K) (phosphothreonine 421/phosphoserine424) detects p70 S6 kinase and p85 S6 kinase only when activated by phosphorylation at Thr421/Ser424 and does not cross-react with other phosphoylated protein kinases.
Spécificité
Does not react with non-phosphorylated S6 kinase (p70S6K) or other phosphorylated protein kinases.
Immunogène
synthetic double phospho-Thr421/Ser424 peptide corresponding to residues around Thr421/Ser424 of human p70S6K.
Application
Anti-phospho-S6-kinase (p70S6K) may be used for immunoblotting at a working dilution of 1:1000 in serum-treated NIH-3T3 cells. For immunoprecipitation, the recommended dilultion is 1:250.
Forme physique
Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Jianying Guo et al.
Oncology letters, 22(2), 630-630 (2021-07-17)
Colorectal cancer (CRC) is the fourth most lethal cancer in the world. Heat shock protein 60 (HSP60), a mitochondrial chaperone that maintains mitochondrial proteostasis, is highly expressed in tumors compared with in paracancerous tissues, suggesting that high HSP60 expression benefits
Roberto Zoncu et al.
Nature reviews. Molecular cell biology, 12(1), 21-35 (2010-12-16)
In all eukaryotes, the target of rapamycin (TOR) signalling pathway couples energy and nutrient abundance to the execution of cell growth and division, owing to the ability of TOR protein kinase to simultaneously sense energy, nutrients and stress and, in
K Hara et al.
The Journal of biological chemistry, 273(23), 14484-14494 (1998-06-11)
The present study identifies the operation of a signal tranduction pathway in mammalian cells that provides a checkpoint control, linking amino acid sufficiency to the control of peptide chain initiation. Withdrawal of amino acids from the nutrient medium of CHO-IR
Hongyu Zhou et al.
Current protein & peptide science, 11(6), 409-424 (2010-05-25)
The mammalian target of rapamycin (mTOR) has attracted substantial attention because of its involvement in a variety of diseases, such as cancer, cardiac hypertrophy, diabetes and obesity. Current knowledge indicates that mTOR functions as two distinct multiprotein complexes, mTORC1 and
Brendan D Manning
The Journal of cell biology, 167(3), 399-403 (2004-11-10)
Proper regulation of the phosphoinositide 3-kinase-Akt pathway is critical for the prevention of both insulin resistance and tumorigenesis. Many recent studies have characterized a negative feedback loop in which components of one downstream branch of this pathway, composed of the
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