5.08838

Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested

• Synonyme(s) : Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested, (2 S)-2-Amino-N-((2 S,3 S,4 R,5 R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
• Formule empirique (notation de Hill): C₂₂H₂₉N₇O₅ · 2HCl
• Numéro CAS: 58-58-2
• Poids moléculaire : 544.43
• Numéro MDL: MFCD00012691
• Code UNSPSC : 12352101

Propriétés

• Pureté: ≥98% (HPLC)
• Niveau de qualité: 200
• Forme: liquid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• Impuretés: ≤10 EU/mg Endotoxin (dry weight)
• Température de stockage: −20°C
• InChI: 1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16?,18+,22+;;/m0../s1
• Clé InChI: MKSVFGKWZLUTTO-USYAMCSGSA-N

Description

Description générale

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor.

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.

Actions biochimiques/physiologiques

Primary Target
RNA

Reversible: yes

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Supplied at 10 mg/ml in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Autres remarques

Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Kaufman, S.H., et al. 1993. Cancer Res.53, 3976.
de la Luna, S. and Ortin, J. 1992. Methods Enzymol.216, 376.
Chow, S.C., et al. 1991. Exp. Cell Res.216, 149.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°C): Not applicable