N1142

Neomycin

solution, suitable for cell culture, BioReagent

• Synonyme(s) : Antibiotic 10676, Neomycin B
• Code UNSPSC : 12352207
• ID de substance PubChem : 24897464
• Nomenclature NACRES : NA.76

Propriétés

• Nom du produit: Neomycin solution, with 10 mg/mL neomycin in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture
• Source biologique: Streptomyces fradiae
• Niveau de qualité: 200
• Stérilité: 0.1 μm filtered
• Gamme de produits: BioReagent
• Forme: liquid
• Puissance: 8-12 mg per mL
• Conditionnement: pkg of 20ML
• Concentration: 10 mg/mL in 0.9% NaCl (neomycin)
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Couleur: clear
• Numéro d'accès UniProt: O14746
• Spectre d'activité de l'antibiotique: Gram-negative bacteria; Gram-positive bacteria
• Mode d’action: protein synthesis | interferes
• Température de stockage: 2-8°C
• Chaîne SMILES: NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O)[C@H]2O[C@@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@@H](CN)[C@@H](O)[C@H](O)[C@H]4N)[C@H]3O)[C@H](N)[C@@H](O)[C@@H]1O
• InChI: 1S/C23H46N6O13/c24-2-7-13(32)15(34)10(28)21(37-7)40-18-6(27)1-5(26)12(31)20(18)42-23-17(36)19(9(4-30)39-23)41-22-11(29)16(35)14(33)8(3-25)38-22/h5-23,30-36H,1-4,24-29H2/t5-,6+,7-,8+,9-,10-,11-,12+,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23+/m1/s1
• Clé InChI: PGBHMTALBVVCIT-VCIWKGPPSA-N
• Informations sur le gène: human ... TERT(7015)

Description

Description générale

Chemical structure: aminoglycoside

Application

Neomycin solution has been used:

• as a supplement of autoclaved acidified water for bone marrow transplantation[1]
• for the selection of stable clones[2]
• as a component of artificial sea water (ASWO) medium to remove bacteria from O. tauri cultures[3]

Actions biochimiques/physiologiques

Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B.[4] It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca²⁺ channels without affecting the Na⁺/Ca²⁺ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria

Attention

This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317,H334
• Conseils de prudence: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter