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P1754

Sigma-Aldrich

TWEEN® 80

TWEEN® 80

viscous liquid

Synonyme(s) :

TWEEN® 80, Mono-oléate de polyoxy-éthylènesorbitane, Mono-oléate de polyéthylène glycol sorbitane, Monooléate de POE (20)-sorbitane, Polysorbate 80

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About This Item

Numéro CAS:
9005-65-6
Numéro MDL:
MFCD00082107
Code UNSPSC :
12161900
Nomenclature NACRES :
NA.21
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

Porcine (tallow)
bovine (tallow)

Niveau de qualité

200

Description

non-ionic

Forme

viscous liquid

Poids mol.

micellar avg mol wt 79,000
average mol wt 1310

Composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

Nombre d'agrégation

60

CMC

0.012 mM (20-25°C)

Viscosité

400-620 mPa.s

Température de transition

cloud point 65 °C

Solubilité

water: soluble, clear to slightly hazy, colorless to yellow

Densité

1.064 g/cm3

HLB

15

Application(s)

cell analysis

Chaîne SMILES 

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

Clé InChI

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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Description générale

Le Tween® 80 est un tensioactif et un détergent non ionique catégorisé comme un ester de sorbitane et d'acide oléique. C'est un composé polyvalent couramment employé dans diverses applications biologiques, notamment la lyse cellulaire, l'isolement des noyaux et le fractionnement cellulaire. Le Tween® 80 permet aussi de stabiliser les protéines et de faciliter l'étude des membranes protéiques. Son efficacité vient de son appartenance au groupe des polysorbates, caractérisé par l'association de chaînes de polyéthylène glycol et d'esters oléiques de sorbitol et de sorbitane. Ces attributs structuraux expliquent les capacités émulsifiantes du Tween® 80 et son vaste éventail d'applications.

Application

Détergent non ionique utilisé pour l'extraction sélective de protéines et l'isolement de noyaux de lignées de cellules de mammifères.
Le TWEEN® 80 a été employé dans une solution de transport de médicament pour des injections spécifiques dans la souris et le rat.[1][2] Il a servi à la détermination de l′activité estérase dans des cellules bactériennes.[3]

Actions biochimiques/physiologiques

Le TWEEN® 80 est un tensioactif du type polysorbate qui contribue à stabiliser les protéines et facilite les études sur les membranes protéiques. Il est régulièrement utilisé dans l′extraction de protéines membranaires et l′isolement de noyaux à partir de cellules.[4][5][6]

Caractéristiques et avantages

  • Excellent agent mouillant et dispersant.
  • Tensioactif extrêmement polyvalent pour vos recherches en biologie cellulaire et en biochimie.

Autres remarques

Pour de plus amples informations sur notre gamme de Produits biochimiques, merci de compléter ce formulaire.

Informations légales

TWEEN is a registered trademark of Croda International PLC

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

>235.4 °F - closed cup

Point d'éclair (°C)

> 113 °C - closed cup

Équipement de protection individuelle

Eyeshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
BCCM6321
BCCM3263
BCCL4939
BCCL8221
BCCL3010

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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SAFC

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European Journal of Pharmacology, 429, 127-127 (2001)
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Questions

1–10 sur 11 questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 réponse

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 réponse

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. This material is free of residual solvents?

    1 réponse

    1. This product is not tested for residual solvents. Should this be a requirement, see product 817061, which is tested for Ethylene Oxide, 1,4-Dioxane, and Ethylene glycol. Please see the link below to review this item:
      https://www.sigmaaldrich.com/product/mm/817061

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  4. What is the boiling point of this specific Tween 80 product?

    1 réponse

    1. This product is not tested for boiling point. However, various reference sources cite a boiling point of approximately 100°C.

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  5. Hello, Is there an expiration date or retest date for this product?

    1 réponse

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 4 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

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  6. Is Product P1754, TWEEN® 80, tested for RNase and DNase activity?

    1 réponse

    1. No. Product P1754 is not tested for contaminating RNase and DNase activity.  Product P5188 is a Molecular Biology grade product that has been tested for DNase and RNase.

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  7. What is the Critical Micelle Concentration (CMC) of Product P1754, TWEEN® 80?

    1 réponse

    1. The CMC value is 13-15 mg/Liter.

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  8. Is Product P1754, TWEEN® 80, cell culture tested?

    1 réponse

    1. No, P1754 is not cell culture tested, but product P4780 is cell culture tested TWEEN 80.

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  9. Is it normal for the color of Product P1754, TWEEN® 80, to change from lot to lot?

    1 réponse

    1. Yes. According to Sigma-Aldrich's specifications, Product No. P1754 can appear bright yellow to yellow with a green cast.  Please see our Specification Sheet for more information.

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  10. Is Product P1754, TWEEN® 80, sterile?

    1 réponse

    1. The product is not sterile. Autoclaving a solution of TWEEN 80 is generally not advised. Sterile filtration is more easily done if the liquid is warmed to about 40°C and alternate portions of hot distilled water and TWEEN 80 are poured through the 0.22 μm filter. The TWEEN 80 will blend and remain in solution.

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