T9378

Trypsin inhibitor

crude powder

• Sinónimos: Trypsin Inhibitor from lima beans
• Número de CAS: 9035-81-8
• Número CE: 232-906-9
• Número MDL: MFCD00082102
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Nombre del producto: Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
• origen biológico: Phaseolus limensis (lima bean)
• Nivel de calidad: 200
• tpo: Type II-L
• Formulario: crude powder
• mol peso: 9 kDa
• solubilidad: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• temp. de almacenamiento: 2-8°C

Descripción

Aplicación

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma[1]
• in the termination of proteolytic digestion in rabbit skeletal muscles[2]
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris[3]
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract[4]

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type[5] enzymes.

Acciones bioquímicas o fisiológicas

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors.[6] It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.[7]

Propiedades físicas

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Definición de unidad

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota de preparación

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Nota de análisis

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Otras notas

View more information on Trypsin Inhibitor.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317,H334
• Consejos de prudencia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)