T9253

Trypsin inhibitor

powder

• Sinónimos: Ovomucoid
• Número de CAS: 9035-81-8
• Número CE: 232-906-9
• Número MDL: MFCD00082102
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Nombre del producto: Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor
• origen biológico: chicken egg white
• Nivel de calidad: 200
• tpo: Type II-O
• Formulario: powder
• mol peso: 14 kDa
• solubilidad: water: soluble 10 g/L
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

25% of ovomucoid comprises carbohydrate.

Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

Aplicación

Trypsin inhibitor from chicken egg white has been used:

• to produce denatured, oxidized and deglycosylated ovomucoid and used to stimulate patient derived cell cultures for mapping T cell epitopes
• to treat nuclei for flow cytometric analysis
• in Holtfreter′s solution, used for the dissociation of planarians into cells

Definición de unidad

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota de análisis

One mg will inhibit 0.8-1.6 mg of tryspin with activity of approx. 10,000 BAEE units per mg protein. May inhibit ≤0.3 mg of chymotrypsin with activity of approx. 40 BTEE units per mg protein.

Otras notas

Ovomucoid, from chicken egg white, does not itself inhibit chymotrypsin. Chymotrypsin inhibition is a measure of ovoinhibitor contamination by method of Feeney, R. E., et al., J. Biol. Chem., 238, 1415 (1963).

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Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317,H334
• Consejos de prudencia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)