T2327
Trypsin inhibitor
lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
Sinónimos:
Kunitz Inhibitor
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About This Item
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product name
Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
origen biológico
Glycine max (soybean)
Línea del producto
BioUltra
Análisis
≥95% (Kunitz inhibitor, SDS-PAGE)
formulario
lyophilized powder
temp. de almacenamiento
2-8°C
Categorías relacionadas
Descripción general
Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site.
Aplicación
Trypsin Inhibitor from Glycine max (soybean) has been used:
- as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestris
- as a standard to compare the trypsin inhibitory activity of the purified protein
- to monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography
- as an trypsin inhibitor
Acciones bioquímicas o fisiológicas
Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.
Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner.
Definición de unidad
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
Nota de preparación
Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.
Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.
Nota de análisis
One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.
Otras notas
View more information on Trypsin Inhibitor.
Aplicación
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1 - Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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We first observed that protein/polysaccharide interaction exhibited noninteracting behavior which makes Bowman-Birk chymotrypsin inhibitor (BBI) always free of complexation, being separated from another protein with similar isoelectric points, Kunitz trypsin inhibitor (KTI). Turbidity titrations showed that the electrostatic attractions were
A continuous fluorometric assay for trypsin based on melittin and the noncovalent-binding-induced pyrene excimer
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Food Chemistry, 155, 24-30 (2014)
Protocolos
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Chromatograms
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