SRP6487

R spondin-1 human

recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE)

• Sinónimos: CRISTIN-3, CRISTIN3, FLJ40906, R-spondin-1, RP11-566C13.1, RSPO, RSPO-1, RSPO1, Rspondin, Rspondin-1
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in HEK 293 cells
• etiqueta: 6-His tagged (C-terminus)
• Análisis: ≥98% (SDS-PAGE)
• formulario: lyophilized
• mol peso: calculated mol wt 27.6 kDa; observed mol wt 40 kDa by SDS-PAGE (reducing) (Ser 21 is the predicted N-terminal .)
• envase: pkg of 10 μg
• impurezas: <1 EU/μg endotoxin (LAL test)
• Nº de acceso UniProt: Q2MKA7
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... RSPO1(284654)

Descripción

Descripción general

R-spondin-1, also known as Roof plate-specific Spondin 1 (RSPO1) and cysteine rich and single thrombospondin domain containing protein 3 (Cristin 3), is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. All R spondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteine rich furin like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin like domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus. Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively.

Aplicación

R spondin-1 human has been used to study the mechanism of action of methylphenidate (a drug to treat attention-deficit hyperactivity disorder), its effect on cell proliferation and maturation.

Acciones bioquímicas o fisiológicas

RSPOs (roof plate-specific spondin) plays a crucial role in embryogenesis and tissue homeostasis. During the developmental stages of ovary, RSPO1 becomes a necessary factor for sex determination and follicular cell progenitor differentiation. The RSPO1 gene expression is also predicted to initiate ovarian cancer. It has a supportive role in cell proliferation, differentiation, and survival. RSPO1 is centrally associated with intestinal stem cell induction.

Forma física

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable