SRP3073

IL-12 human

recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinónimos: CTL maturation factor (TCMF),, Cytotoxic lymphocyte maturation factor (CLMF), NKSF, TSF
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in CHO cells
• Ensayo: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Formulario: lyophilized
• potencia: ≤1.0 ng/mL ED₅₀
• mol peso: 40 kDa
• envase: pkg of 10 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• Nº de acceso UniProt: P29459; P29460
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... IL12A(3592)

Descripción

Descripción general

IL-12 (interleukin 12) is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Recombinant Human IL-12 is a 75kDa heterodimeric glycoprotein consisting of disulfide-linked 35kDa (p35) and 40kDa (p40) subunits (503 total amino acid residues).

Acciones bioquímicas o fisiológicas

IL-12 (interleukin 12) is a proinflammatory cytokine and plays an important role in protection against pathogens.[1] IL-12 is a potent regulator of cell mediated immune responses and it induces IFN (interferon)-γ production by NK (natural killer) and T cells.[2][3] Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-γ producing Th1 (T helper) cells. Polymorphisms in the IL-12 gene are associated with risk of rheumatoid arthritis.[4] It also exhibits anti-tumor activities by enhancing Th1 adaptive immunity and cytotoxic T lymphocyte functions.[5]

Secuencia

p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Forma física

Lyophilized from 0.5x PBS, pH 7.2.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2-7.4 to a concentration of 0.2 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable