H7666

Interleukin-12 human

HumanKine^®, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin, tested

• Sinónimos: IL-12
• Número MDL: MFCD00212975
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• Análisis: ≥95% (SDS-PAGE)
• formulario: lyophilized
• potencia: ≤2 ng/mL EC₅₀
• mol peso: dimer 57 kDa (glycosylated)
• envase: pkg of 10 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• temp. de almacenamiento: −20°C

Descripción

Aplicación

Interleukin-12 (IL-12) human has been used to study IL-12 mediated enhancement of IL-12Rβ2 (IL-12 receptor) expression.

Acciones bioquímicas o fisiológicas

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Forma física

lyophilized from a 0.2 μm filtered solution of PBS

Nota de análisis

The specific activity was determined by the dose-dependent release of IFN-γ from the human NK92 cell line in presence of 20 ng/mL rIL-2.

Información legal

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable