SRP0710

EGFR (T790M C797S L858R), Active

PRECISIO ^®Kinase, recombinant

• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• recombinante: expressed in baculovirus infected Sf9 cells
• Análisis: 70-95% (SDS-PAGE)
• formulario: frozen liquid
• actividad específica: 34-46 nmol/min·mg (radiometric)
• mol peso: ~89-100 kDa
• concentración: 0.1 μg/μL
• nº de acceso: NM_005228
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... EGFR(1956)

Descripción

Aplicación

Useful for kinase assay.

Acciones bioquímicas o fisiológicas

Recombinant human EGFR (T790M C797S L858R) (668-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival ⁽¹⁾. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms ⁽²⁾. Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.

Reconstitución

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Almacenamiento y estabilidad

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Información legal

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable