SRP6404

EGFR/ErbB1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinónimos: ERBB, ERBB1, HER1, PIG61, mENA
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in HEK 293 cells
• etiqueta: 6-His tagged (C-terminus)
• Ensayo: ≥95% (SDS-PAGE)
• Formulario: lyophilized
• mol peso: calculated mol wt 69.5 kDa; observed mol wt 110-115 kDa (DTT-reduced.Protein migrates due to glycosylation.)
• envase: pkg of 10 μg
• impurezas: <1 EU/μg endotoxin (LAL test)
• Nº de acceso UniProt: P00533
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... EGFR(1956)

Descripción

Descripción general

EGFR (epidermal growth factor receptor) belongs to the ErbB family of receptor tyrosine kinases.[1] It is also known as ErbB1/HER1 (human epidermal growth factor receptor). It is located on human chromosome 7p11.2.[2] EGFR has a large extracellular ligand-binding domain, a single transmembrane segment, an intracellular juxtamembrane segment, a tyrosine kinase domain and a carboxy-terminal tail.[1]

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. (provided by RefSeq)

Acciones bioquímicas o fisiológicas

EGFR (epidermal growth factor receptor) plays an important role in ligand binding, dimerization and autophosphorylation.[1] It also plays an important role in several procarcinogenic cancers like gastric cancer. EGFR controls various functions of epithelial cell. It modulates the actions in bacterial infection and is associated with the initiation of macrophage.[3]

Forma física

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable