Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

SHC003V

Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles

Green fluorescent protein marker to monitor transduction efficiency

Sinónimos:

MISSION®, MISSION® TurboGFP™ Control Transduction Particles

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización

Seleccione un Tamaño

SHC003VN
385,00 €

385,00 €

Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Solicitar un pedido a granelSolicitar más información
Todas las fotos(1)

Seleccione un Tamaño

Cambiar Vistas
SHC003VN
385,00 €

About This Item

Código UNSPSC:
41106609
NACRES:
NA.51

385,00 €

Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Solicitar un pedido a granelSolicitar más información

Nivel de calidad

100
200

Línea del producto

MISSION®

concentración

≥1x106 VP/ml (via p24 assay)

técnicas

capture ELISA: 106 TU/mL using p24

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Categorías relacionadas

Descripción general

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from the copepode Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003). It is a positive control to monitor transduction efficiency.[1]

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Aplicación

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.[2][3]

Información legal

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.

Opcional

Referencia del producto
Descripción
Precios

SHC002

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes

SHC001V

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC002V

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes

SHC004

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC004V

Partículas de control de transducción de shRNA MISIÓN® pLKO.1-puro TurboGFP™, shRNA sequence targeting tGFP

SHM01

ExpressMag® Super Magnetic Kit, Increases transduction efficiency

SHM02

ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency

SHC012V

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles, Contains a gene encoding TagRFP

SHC014V

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Transduction Particles, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
06072112MN
09041810MN

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Ruoxiang Wang et al.
The Prostate, 80(3), 274-283 (2019-12-18)
We previously determined that cancer-stromal interaction was a direct route to tumor cell heterogeneity progression, since cancer-stromal cell fusion in coculture resulted in the creation of heterogeneous clones of fusion hybrid progeny. In this report, we modified the cancer-stromal coculture
A high-content image-based method for quantitatively studying context-dependent cell population dynamics.
Garvey CM
Scientific Reports, 6, 29752-29752 (2016)
Esperanza Martín-Sánchez et al.
PloS one, 9(11), e112148-e112148 (2014-11-12)
Currently, there is no efficient therapy for patients with peripheral T cell lymphoma (PTCL). The Proviral Integration site of Moloney murine leukemia virus (PIM) kinases are important mediators of cell survival. We aimed to determine the therapeutic value of PIM
Two-Photon Microscopy Analysis of Gold Nanoparticle Uptake in 3D Cell Spheroids.
Rane TD and Armani AM
PLoS ONE, 11(12) (2016)
Chi-Li Chiu et al.
Scientific reports, 6, 22435-22435 (2016-03-05)
The androgen receptor (AR) pathway plays a central role in prostate cancer (PCa) growth and progression and is a validated therapeutic target. In response to ligand binding AR translocates to the nucleus, though the molecular mechanism is not well understood.
Ver todos los artículos relacionados

Artículos

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Protocolos

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Preguntas

Revisiones

Sin puntuación

Filtros activos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico