SHC004
MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA
shRNA sequence targeting tGFP
Sinónimos:
MISSION® Control Vectors
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 pkg | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 408,00 € |
Acerca de este artículo
Concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
408,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
General description
The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
MISSION® pLKO.1-puro TurboGFP™ shRNA control plasmid DNA has been used as lentiviral transduction and RNA interference assay.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
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Este artículo | |||
|---|---|---|---|
| description shRNA sequence targeting tGFP | description Targets no known mammalian genes | description shRNA sequence targeting eGFP | description shRNA sequence targeting tGFP |
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration ≥1x106 VP/ml (via p24 assay) |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Clase de almacenamiento
12 - Non Combustible Liquids
¿Qué está pasando?
WGK 1
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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