R7397

ProtectRNA^™ RNase Inhibitor 500× Concentrate

RNase inhibitor for in situ hybridization assays

• Sinónimos: Ribonuclease inhibitor ProtectRNA^™
• Código UNSPSC: 41106300
• NACRES: NA.52

Propiedades

• Nivel de calidad: 200
• Formulario: liquid
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Not recommended in systems where other enzymatic activity is required.

ProtectRNA^™ ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.

Aplicación

ProtectRNA^™ RNase Inhibitor 500× Concentrate has been used in:

• RNA extraction[1]
• tissue sectioning and staining for laser capture microscopy[2]
• the isolation of blood vessels from hippocampi[3]

ProtectRNA^™ RNase Inhibitor 500× Concentrate is an RNase inhibitor.[4][5][6]

A potent inhibitor of most nucleic acid binding enzymes, and thus useful as an RNase inhibitor. Especially useful when performing in situ hybridization. If it is added to all aqueous solutions used, it eliminates the need for special glassware washing and after-wash treatments. The 500x concentrate is economical; 2 ml treats 1,000 ml of solution. Not recommended in systems where other enzymatic activity is required.

Características y beneficios

• The 500× concentrate is economical
• Eliminates the need for special dishwashing and“after washing” cleaning and treatments.

Cantidad

2mL treats 1,000ml of solution.

Otras notas

This product is for R&D use only, not for drug, household, or other uses.

Información legal

ProtectRNA is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Pictogramas: GHS02,GHS05
• Palabra de señalización: Warning
• Frases de peligro: H226,H290
• Consejos de prudencia: P210 - P233 - P234 - P240 - P241 - P242
• Clasificaciones de peligro: Flam. Liq. 3 - Met. Corr. 1
• Código de clase de almacenamiento: 3 - Flammable liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): 140.0 °F - closed cup
• Punto de inflamabilidad (°C): 60 °C - closed cup