R1158

Ribonuclease Inhibitor

recombinant, expressed in E. coli, 20-40 units/μL

• Sinónimos: RNAse inhibitor
• Número MDL: MFCD00132187
• Código UNSPSC: 12352200
• NACRES: NA.52

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 300
• grado: for molecular biology
• Formulario: buffered aqueous solution
• mol peso: ~50 kDa
• concentración: 20-40 units/μL
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).

Aplicación

Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:

• cDNA synthesis from mRNA
• reverse transcriptase polymerase chain reaction (RT-PCR)
• in vitro transcription and translation
• mRNA protection from degradation
• formaldehyde cross-linked lncRNA purification
• RNA-fluorescence in situ hybridization (FISH)

Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Acciones bioquímicas o fisiológicas

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

Componentes

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Definición de unidad

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Forma física

Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.

Otras notas

• Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
• This product is for R&D use only, not for drug, household, or other uses.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable