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M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

Sinónimos:

MgCl2, dichloromangesium, magnesium chloride

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About This Item

Fórmula lineal:
MgCl2
Número de CAS:
7786-30-3
Peso molecular:
95.21
Número MDL:
MFCD00011106
Código UNSPSC:
12352302
ID de la sustancia en PubChem:
24896582
NACRES:
NA.52

grado

for molecular biology

Nivel de calidad

300

esterilidad

non-sterile; 0.2 μm filtered

formulario

liquid

concentración

1.00 M±0.01 M

técnicas

DNA sequencing: suitable

impurezas

DNAse, none detected
 NICKase, none detected
 Protease, none detected
 RNAse, none detected

solubilidad

water: soluble

densidad

1.080 g/cm3

trazas de catión

heavy metals (as Pb): ≤5 ppm

idoneidad

suitable for electrophoresis
suitable for molecular biology

aplicaciones

cell analysis

actividad extraña

DNase, RNase, NICKase and protease, none detected

cadena SMILES

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

Clave InChI

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Descripción general

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

Aplicación

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -
  • to prepare buffer to lyse cells for immunoprecipitation
  • as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.
  • in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.
  • It has been used to protect the liver from oxidative damage.
  • to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Características y beneficios

  • Available as a Ready-to-use solution which reduces the need for preparation time.
  • Heavy metals (as Pb): ≤5 ppm
  • Free from DNase, RNase, NICKase and protease

Componentes

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Otras notas

For additional information on our range of Biochemicals, please complete this form.

producto comparable

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protective Effects of Magnesium Chloride on Liver Enzymes and Biomarkers of Oxidative Stress in high fat diet fed Rats
Mohammed et, al
Nigerian Journal of Physiological Sciences : Official Publication of the Physiological Society of Nigeria, 34(2), 149-157 (2019)
Yelena Y Grinberg et al.
Journal of neurochemistry, 122(1), 221-229 (2012-04-25)
Spreading depression (SD), the likely cause of migraine aura and perhaps migraine, is triggered by widespread and unfettered neuronal hyperexcitability. Migraine and the initiating hyperexcitability of seizure, which involve oxidative stress (OS), are likely interrelated. Environmental enrichment (EE) decreases seizure
M J Mc Garrigle et al.
European cells & materials, 31, 323-340 (2016-05-25)
Extracellular matrix (ECM) stiffness and cell density can regulate osteoblast differentiation in two dimensional environments. However, it is not yet known how osteoblast-osteocyte differentiation is regulated within a 3D ECM environment, akin to that existing in vivo. In this study
Antimo Migliaccio et al.
Methods in molecular biology (Clifton, N.J.), 776, 361-370 (2011-07-29)
Much evidence indicates that, with few exceptions, non-genomic actions of steroids are mediated by receptors universally known as nuclear receptors. Steroid receptors do not exhibit intrinsic tyrosine kinase activity. Nevertheless, they stimulate different signaling pathways in cytoplasm of target cells
Hiroaki Eshima et al.
Journal of applied physiology (Bethesda, Md. : 1985), 128(5), 1153-1162 (2020-03-28)
Obesity and aging reduce skeletal muscle contractile function. However, it remains unclear whether obesity additively promotes muscle contractile dysfunction in the setting of aging. In this study, we investigated skeletal muscle contractile function ex vivo and intracellular Ca2+ release in
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Protocolos

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

Enzymatic Assay of Hexokinase

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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