P5400
Pectinesterase from orange peel
lyophilized powder, ≥150 units/mg protein
Sinónimos:
Pectin methylesterase, Pectin pectylhydrolase
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About This Item
Productos recomendados
formulario
lyophilized powder
actividad específica
≥150 units/mg protein
composición
Protein, 20-50% biuret
características de los productos alternativos más sostenibles
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
categoría alternativa más sostenible
, Enabling
temp. de almacenamiento
2-8°C
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Categorías relacionadas
Descripción general
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Aplicación
Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene.
The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.
Acciones bioquímicas o fisiológicas
Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.
Definición de unidad
One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.
Forma física
Contains (NH4)2SO4 and sodium chloride
Nota de análisis
Protein determined by biuret.
Otras notas
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Doungla E Ngouémazong et al.
Carbohydrate research, 348, 69-76 (2012-01-03)
The influence of the degree and pattern of methylesterification (DM and PM, respectively) on the stiffness of Ca(2+)-pectin gels is extensively examined, at various Ca(2+) concentrations. Accordingly, a highly methyl-esterified pectin was selectively de-esterified using NaOH, plant or fungal pectin
Ida Barbara Reca et al.
Plant molecular biology, 79(4-5), 429-442 (2012-05-23)
A pectin methylesterase inhibitor (SolyPMEI) from tomato has been identified and characterised by a functional genomics approach. SolyPMEI is a cell wall protein sharing high similarity with Actinidia deliciosa PMEI (AdPMEI), the best characterised inhibitor from kiwi. It typically affects
Yuri L Dorokhov et al.
PLoS pathogens, 8(4), e1002640-e1002640 (2012-04-13)
Many plants release airborne volatile compounds in response to wounding due to pathogenic assault. These compounds serve as plant defenses and are involved in plant signaling. Here, we study the effects of pectin methylesterase (PME)-generated methanol release from wounded plants
Mélanie L'Enfant et al.
International journal of biological macromolecules, 132, 1140-1146 (2019-04-13)
The discovery of molecules that can inhibit the action of phytopathogens is essential to find alternative to current pesticides. Pectin methylesterases (PME), enzymes that fine-tune the degree of methylesterification of plant cell wall pectins, play a key role in the
Kerstin Müller et al.
Plant physiology, 161(1), 305-316 (2012-11-07)
The methylesterification status of cell wall homogalacturonans, mediated through the action of pectin methylesterases (PMEs), influences the biophysical properties of plant cell walls such as elasticity and porosity, important parameters for cell elongation and water uptake. The completion of seed
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