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Merck

H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max

liquid, sterile-filtered, BioReagent, suitable for hybridoma

Sinónimos:

Histopaque 1077

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100 ML
90,70 €
500 ML
302,00 €

90,70 €


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100 ML
90,70 €
500 ML
302,00 €

About This Item

Código UNSPSC:
12352201
NACRES:
NA.75

90,70 €


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esterilidad

sterile-filtered

Línea del producto

BioReagent

Formulario

liquid

técnicas

cell culture | hybridoma: suitable
single cell analysis: suitable

impurezas

endotoxin, tested

densidad

1.077 at 25 °C (relative density)(lit.)

temp. de almacenamiento

2-8°C

Descripción general

Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3.[1] This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Aplicación

Histopaque®-1077 Hybri-Max has been used to isolate:
  • peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation[2]
  • immune cells (lymphocytes) from peripheral blood[3]
  • PBMCs from blood[4]

Información legal

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Carla Oseroff et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(39), 13980-13985 (2005-09-21)
We have analyzed by ex vivo ELISPOT the anti-vaccinia cytotoxic T lymphocyte responses of peripheral blood mononuclear cells from humans vaccinated with Dryvax vaccine. More than 6,000 peptides from 258 putative vaccinia ORFs predicted to bind the common molecules of
Mauricio S Caetano et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(24), 7576-7584 (2019-09-22)
Radiotherapy (RT) traditionally has been used for local tumor control in the treatment of cancer. The recent discovery that radiotherapy can have anticancer effects on the immune system has led to recognition of its ability to sensitize the tumor microenvironment
Janet E Alexander et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 73(6), 720-728 (2017-11-11)
As in human populations, advances in nutrition and veterinary care have led to an increase in the lifespan of companion animals. Detrimental physiological changes occurring later in life must be understood before interventions can be made to slow or reduce
Elliott D SoRelle et al.
eLife, 10 (2021-01-28)
Lymphoblastoid cell lines (LCLs) are generated by transforming primary B cells with Epstein-Barr virus (EBV) and are used extensively as model systems in viral oncology, immunology, and human genetics research. In this study, we characterized single-cell transcriptomic profiles of five
Joshua E Messinger et al.
mBio, 10(4) (2019-07-04)
Deciphering the molecular pathogenesis of virally induced cancers is challenging due, in part, to the heterogeneity of both viral gene expression and host gene expression. Epstein-Barr virus (EBV) is a ubiquitous herpesvirus prevalent in B-cell lymphomas of immune-suppressed individuals. EBV

Protocolos

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Preguntas

1–2 de 2 Preguntas  
  1. What is the formulation for H8889 Histopaque-1077 Hybri-Max?

    1 respuesta
    1. The formulation for H8889 Histopaque-1077 Hybri-Max consists of 5.7% polysucrose 400 and 9% sodium diatrizoate.

      ¿Le ha resultado útil?

  2. Is there a product in your inventory that is comparable to the Corning product 25-072-CV?

    1 respuesta
    1. Upon comparison of the formulation and product description, the comparable product from MilliporeSigma is identified as product number H8889. Both products, 25-072 and H8889, have a density of 1.077 g/ml and share the same formulation components, including Diatrizoic acid at 96.219 and Polysucrose 400 at 61.363.

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