Percoll and Percoll Plus are manufactured by Cytiva. The Product Information Sheet for this product provides a basic protocol and references for common cell types. Additional references and mathematical models can be found on the Cytiva website.
Typically, references identify a specific density for each cell type or molecule of interest. Instructions for preparing a specific density are included in the product information sheet or a similar document on the Cytiva website. The references will mention the G force to be used, as it accurately defines the centrifuge conditions. If revolutions per minute (RPM) is stated, the radius of the centrifuge affects the outcome. Nomogram tables are available for converting G force to RPM. To find a nomogram table, search for "nomogram" in the document titled "Centrifugal Filters: Guide to Converting g-Force to RPM." Instructions for use are included to convert G force to the appropriate RPM setting for a specific centrifuge.
P4937
Percoll®
pH 8.5-9.5 (25 °C), suitable for cell culture
Sinónimos:
Medio gradiente de densidad
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| Talla/SKU | Disponibilidad | Precio |
|---|
Acerca de este artículo
sterility
aseptically filled
Quality Segment
form
colloidal
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
pH
8.5-9.5 (25 °C)
density
1.13 g/mL±0.005 g/mL at 25 °C (lit.)
storage temp.
2-8°C
General description
Application
Features and Benefits
- La baja osmolalidad permite un ajuste preciso a las condiciones fisiológicas sin interferencias significativas del medio.
- Compatible con células y virus vivos y permite la separación y la recuperación de sistemas intactos, totalmente activos.
- Impermeable a las membranas biológicas lo que no provoca ningún cambio en la densidad de flotación de las partículas durante la centrifugación.
- La formación espontánea del gradiente durante la centrifugación permite mezclar grandes volúmenes de muestra en los tubos de centrífuga.
- La baja viscosidad provoca una rápida formación de gradientes y separación de partículas.
- La mayoría de las partículas biológicas que tienen valores de coeficiente de sedimentación superiores a 60S pueden aislarse satisfactoriamente en gradientes de Percoll.
Legal Information
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Este artículo | |||
|---|---|---|---|
| description pH 8.5-9.5 (25 °C), suitable for cell culture | description pH 8.5-9.5 (20 °C) | description suitable for plant cell culture | description Type 400, 20% in H2O |
| form colloidal | form colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) ) | form colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) | form liquid |
| density 1.13 g/mL±0.005 g/mL at 25 °C (lit.) | density - | density - | density 1.055-1.075 g/mL |
| sterility aseptically filled | sterility aseptically filled | sterility aseptically filled | sterility 0.2 μm filtered |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | plant: suitable | technique(s) titration: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Clase de almacenamiento
12 - Non Combustible Liquids
¿Qué está pasando?
nwg
EPI (equipos de protección individual)
Eyeshields, Gloves
Punto de inflamación (°C)
Not applicable
Punto de inflamación (°F)
Not applicable
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Further to the above question: "When using Product P1644, Percoll®, what density should I use for my particular system?", when I review the sheet for granulocytes and RBCs it gives a centrifugation speed as "*" . What does this mean?
1 respuesta-
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When using Product P1644, Percoll®, what density should I use for my particular system?
1 respuesta-
The product information sheet (under Documents, above) has a table of densities for different organelles and cell types.
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Can Percoll®, Product P1644, be autoclaved?
1 respuesta-
Percoll can be autoclaved, but this must be done without any salts or sucrose present.
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Can you help me calculate gradients for Percoll®, Product P1644?
1 respuesta-
The product information sheet (under Documents, above) has an equation for calculating density of a given solution.
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What is the difference between Percoll® Products P4937 and P1644?
1 respuesta-
Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.
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Is there a "best" salt solution to use with Product P1644, Percoll®?
1 respuesta-
Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested. Sucrose is included to prevent aggregation. You can find detailed preparation instructions on the product information sheet (under Documents, above) for this product.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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