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Merck

10831

Sigma-Aldrich

Histopaque®-1083

sterile-filtered, density: 1.083 g/mL

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

esterilidad

sterile-filtered

formulario

liquid

caducidad

Expiry date on the label.

impurezas

endotoxin, tested

densidad

1.083 g/mL

aplicaciones

hematology
histology

temp. de almacenamiento

2-8°C

Descripción general

Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Aplicación

Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Características y beneficios

Histopaque®-1083 offers numerous advantages, such as:

  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Principio

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Información legal

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

Protocolos

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

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