Unfortunately, the solution stability of this material at 2-8°C has not been determined. However, solutions may be stored in aliquots at -20°C for up to 3 months.
H2625
Hemoglobin from bovine blood
suitable for protease substrate, substrate powder
Sinónimos:
Bovine hemoglobin, Hb, Methemoglobin
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About This Item
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Productos recomendados
origen biológico
bovine blood
Formulario
substrate powder
mol peso
Mr ~64500
técnicas
activity assay: suitable
solubilidad
H2O: soluble 20 mg/mL
idoneidad
suitable for protease substrate
temp. de almacenamiento
2-8°C
Información sobre el gen
cow ... HBA1(281221) , HBB(280813) , HBE2(513240) , HBE4(513108) , HBG(511735) , alpha globin(512439)
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Descripción general
Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%.[1] Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.[2]
Aplicación
Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay.[3][4] It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.[5]
The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.
Acciones bioquímicas o fisiológicas
Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin.[6] Bovine hemoglobin interacts with synthetic and azo dyes.[2] Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.[7]
Oxygen transporter, NO scavenger
Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.
Precaución
Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.
Nota de preparación
Prepared from washed, lysed and dialyzed erythrocytes.
Aplicación
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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A study of the interaction of bovine hemoglobin with synthetic dyes using spectroscopic techniques and molecular docking
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Protocolos
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.
Chromatograms
application for HPLC-
If made up in solution and stored at 2-8C, how long can this product last for?
1 respuesta-
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Is this product suitable for mammalian cell culture?
1 respuesta-
H2625 is specified to be suitable as a substrate in a protease assay. It is not evaluated for cell culture use.
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Do you have any information on purity of this product?
1 respuesta-
This product has not been tested for purity. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/400/152/h2500pis.pdf¿Le ha resultado útil?
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