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CPISO

Chloroplast Isolation Kit

isolation of intact chloroplasts from leaves

Sinónimos:

Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation

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Talla/SKUDisponibilidadPrecio
1 kit
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938,00 €

Acerca de este artículo

NACRES:
NA.32
UNSPSC Code:
12352200

938,00 €


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Quality Segment

technique(s)

centrifugation: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.

Application

Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.
The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.

Features and Benefits

  • Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
  • Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
  • Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
  • Convenient format - Saves time and reduces waste

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CELLYTPN1G2N70PEROX1
description

isolation of intact chloroplasts from leaves

description

For plant leaves

description

sufficient for 70 purifications

description

isolate peroxisomes from tissues and cells

technique(s)

centrifugation: suitable

technique(s)

ChIP: suitable

technique(s)

DNA purification: suitable

technique(s)

fractionation: suitable, centrifugation: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS & Precios

  • Chloroplast Isolation Buffer 5x (CIB) 500 mL
    SDS

  • Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 g
    SDS

  • Chloroplast Isolation Buffer 5x (CIB) 4 ea
    SDS

  • Percoll®, pH 8.5-9.5 (25 °C), suitable for cell culture 100 mL
    SDS

Clase de almacenamiento

10 - Combustible liquids



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Preguntas

  1. How many samples can be processed with this kit?

    1 respuesta
    1. This kit provides sufficient reagents to perform 18 chloroplast isolation procedures, using 30 grams of starting plant tissue, as described in the protocol. Please see the link below to review the product datasheet:_x000D_
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/229/cpisopis-ms.pdf

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