This kit provides sufficient reagents to perform 18 chloroplast isolation procedures, using 30 grams of starting plant tissue, as described in the protocol. Please see the link below to review the product datasheet:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/229/cpisopis-ms.pdf
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 938,00 € |
Acerca de este artículo
NACRES:
NA.32
UNSPSC Code:
12352200
938,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleGeneral description
The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.
Application
Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.
The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.
Features and Benefits
- Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
- Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
- Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
- Convenient format - Saves time and reduces waste
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Este artículo | |||
|---|---|---|---|
| description isolation of intact chloroplasts from leaves | description For plant leaves | description sufficient for 70 purifications | description isolate peroxisomes from tissues and cells |
| technique(s) centrifugation: suitable | technique(s) ChIP: suitable | technique(s) DNA purification: suitable | technique(s) fractionation: suitable, centrifugation: suitable |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in wet ice |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS & Precios
Clase de almacenamiento
10 - Combustible liquids
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