The correct volume is 1mL per mouse spleen, or 100-200 million cells, for use in hybridoma protocols.
R7757
Red Blood Cell Lysing Buffer Hybri-Max™
Liquid, sterile-filtered, suitable for hybridoma
Sinónimos:
RBC Lysing Buffer
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
100 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 60,30 € |
Acerca de este artículo
UNSPSC Code:
12352207
NACRES:
NA.75
60,30 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleNombre del producto
Red Blood Cell Lysing Buffer Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma
sterility
sterile-filtered
Quality Segment
form
liquid
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
Application
Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.
Biochem/physiol Actions
Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.
Preparation Note
Contains 8.3 g/L ammonium chloride in 0.01 M Tris-HCl buffer.
Other Notes
Note: This product is intended for the removal of red blood cells from mice. This product may not be appropriate for the lysis of red blood cells of other animals. The suitability of the product in any application other than mouse splenocytes must be determined by the researcher.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
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Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | hybridoma: suitable | technique(s) - | technique(s) cell culture | hybridoma: suitable, single cell analysis: suitable | technique(s) cell culture | hybridoma: suitable |
| Quality Level 400 | Quality Level - | Quality Level 400 | Quality Level 500 |
| form liquid | form solution | form liquid | form liquid |
| impurities endotoxin, tested | impurities - | impurities endotoxin, tested | impurities - |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility sterile-filtered |
RBC lysing buffer, RBC solubilization buffer | - | density gradient, gradient centrifugation, lymphocyte purification reagent | monoclonal antibody production medium, mAb production medium, biotechnology grade hybridoma medium |
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Listados normativos
Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.
12125-02-9
CAS No.
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I will be using your RBC cell lysing buffer cat# R7757. The protocol on your web site mentions to add 1mL of the lysis buffer to a cell pellet representing 1 spleen. Is this correct? That just seems low to me. Please let me know.
1 respuesta-
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Since you do not recommend Product R7757, Red Blood Cell Lysing Buffer Hybri-Max™, for treating whole blood, what protocol and products do you recommend for red cell lysis in a sample of whole blood?
1 respuesta-
The following is a suggested protocol:1. Centrifuge to remove plasma. A volume of 0.2 mL of whole blood will yield approximately 0.1 mL of packed cells. Add 0.1ml of 1X buffer such as HBSS (Hanks' balanced salt solution, Product No. H1641) or DPBS (Dulbecco's phosphate buffered saline solution, Product No. D1283), to 0.1 mL of the pelleted cells and resuspend the pellet.2. Add 1-2 mls of 0.1X diluent (or water) and quickly mix cells using the same pipette.3. After 15 seconds, quickly add a volume of 2X diluent in the amount used in step 2 and mix thoroughly. Dilute further with 1X diluent. Wash cells once with buffer.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is the BSA standard supplied with Product B6916, Bradford Reagent?
1 respuesta-
No, we do not supply the BSA standard with this reagent. We recommend using one of the following products: Protein Standard (BSA) Solution, (2 mg/mL), Product No. P0834 or Protein Standard (BSA) Solution, (1 mg/mL), Product No. P0914, if lower concentrations of protein are to be measured.
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In step 3 of your usage sheet for Product R7757, Red Blood Cell Lysing Buffer Hybri-Max™, you say to dilute this buffer with medium or "salt solution". What salt solution is appropriate?
1 respuesta-
Any balanced salt solution such as DPBS (Dulbecco's phosphate buffered saline) or HBSS (Hank's Balanced Salt Solution) may be used to dilute the buffer when following this procedure.
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Can Product R7757, Red Blood Cell Lysing Buffer Hybri-Max™, be used to lyse cells that are trapped in whole tissue preparations?
1 respuesta-
Unfortunately, we have not verified the suitability of this lysis buffer for using with tissue samples and do not have a protocol.
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