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Merck

A9972

Sigma-Aldrich

α-Amylase from human pancreas

greener alternative

lyophilized powder, ≥100 units/mg protein

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

origen biológico

human pancreas

formulario

lyophilized powder

actividad específica

≥100 units/mg protein

purificado por

2× crystallization

características de los productos alternativos más sostenibles

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Nº de acceso UniProt

categoría alternativa más sostenible

temp. de almacenamiento

−20°C

Información sobre el gen

Descripción general

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Acciones bioquímicas o fisiológicas

α-Amylase is composed of 496 amino acids in a single polypeptide chain. It hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.

Definición de unidad

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Forma física

Lyophilized from Tris buffer containing NaCl and CaCl2.

Nota de preparación

Prepared by modified method of Levitzki et al.

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Evaluation of an automated system for amylase detection in forensic samples
Vela GD, et al.
International Congress Series, 1288, 636-638 (2006)
Yong Qin Koh et al.
Heliyon, 8(8), e10131-e10131 (2022-08-23)
Carbohydrate digestibility is a key determinant for elevated postprandial hyperglycemia (PPHG). Apart from dietary restrictions, one of the strategies to reduce PPHG is to limit the activity of carbohydrate digestive enzymes within the gastrointestinal tract in order to reduce monosaccharide
Lawrence Quarino et al.
Journal of forensic sciences, 50(4), 873-876 (2005-08-05)
An ELISA method for the detection of salivary amylase in dried stains using a monoclonal anti-human salivary amylase antibody was developed. Studies demonstrated the assay to be sensitive down to 0.0002 Sigma units and showed a linear response between absorbance
G D Brayer et al.
Protein science : a publication of the Protein Society, 4(9), 1730-1742 (1995-09-01)
The structure of human pancreatic alpha-amylase has been determined to 1.8 A resolution using X-ray diffraction techniques. This enzyme is found to be composed of three structural domains. The largest is Domain A (residues 1-99, 169-404), which forms a central
Benjamin C M Pang et al.
Journal of forensic sciences, 53(5), 1117-1122 (2008-07-22)
The RSID-saliva test and the SALIgAE-saliva test are two recently developed forensic saliva detection kits. In this study, we compared the sensitivity and the specificity of the two test kits with the Phadebas amylase test by analyzing amylases from various

Protocolos

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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