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Merck

P1254

Sigma-Aldrich

Brij® L23

suitable for Stein-Moore chromatography

Sinónimos:

Brij 35, C12E23, Polyoxyethylene (23) lauryl ether

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500 G
73,70 €
5 KG
481,00 €

73,70 €


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500 G
73,70 €
5 KG
481,00 €

About This Item

Número de CAS:
Número MDL:
Código UNSPSC:
12161900
ID de la sustancia en PubChem:
NACRES:
NA.21

73,70 €


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descripción

non-ionic

Nivel de calidad

Formulario

solid

mol peso

micellar avg mol wt 48,000
estimated mol wt 1198

número de agregación

20-40

CMC

91 μM

temperatura de transición

cloud point >100 °C

HLB

16.9

idoneidad

suitable for Stein-Moore chromatography

cadena SMILES

CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO

InChI

1S/C58H118O24/c1-2-3-4-5-6-7-8-9-10-11-13-60-15-17-62-19-21-64-23-25-66-27-29-68-31-33-70-35-37-72-39-41-74-43-45-76-47-49-78-51-53-80-55-57-82-58-56-81-54-52-79-50-48-77-46-44-75-42-40-73-38-36-71-34-32-69-30-28-67-26-24-65-22-20-63-18-16-61-14-12-59/h59H,2-58H2,1H3

Clave InChI

IEQAICDLOKRSRL-UHFFFAOYSA-N

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Aplicación

Non-ionic detergent. Useful for the extraction of membrane proteins. Typically used in the range of 0.1-1%.

Información legal

Brij is a registered trademark of Croda International PLC

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

>300.2 °F - open cup

Punto de inflamabilidad (°C)

> 149 °C - open cup

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves


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Two forms of an acid proteinase have been purified from human erythrocyte membranes by a simple method involving selective extraction with 0.5% Brij 35, affinity chromatography on pepstatin A-Sepharose 4B, and chromatography on Sephacryl S-200 and DEAE-Sephadex G-100. One species
T Tobimatsu et al.
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Recombinant adenosylcobalamin-dependent diol dehydratase of Klebsiella oxytoca overexpressed in Escherichia coli was purified to homogeneity. The enzyme has a low solubility and was extracted from the crude membrane fraction with 1% Brij 35 in a high recovery. Subsequent chromatography on
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Preguntas

1–3 de 3 Preguntas  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 respuesta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 respuesta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. How to prepare a 1% solution of Brij 35?

    1 respuesta
    1. To prepare a 1% solution of Brij 35, first verify the concentration of the Brij 35 solution. Once the concentration is known, can use water as the diluent to prepare the 1% solution of Brij 35. If the Brij 35 solution is not already at the desired 1% concentration, dilute it accordingly with water.

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