K1637

Canamicina sulfate from Streptomyces kanamyceticus

meets USP testing specifications, powder

• Sinónimos: Canamicina A, Canamicina sulfate salt
• Fórmula empírica (notación de Hill): C₁₈H₃₆N₄O₁₁ · H₂O₄S
• Número de CAS: 25389-94-0
• Peso molecular: 582.58
• Número CE: 246-933-9
• Número MDL: MFCD00070253
• Código UNSPSC: 51281654
• ID de la sustancia en PubChem: 24896209
• NACRES: NA.76

Propiedades

• origen biológico: Streptomyces kanamyceticus
• Nivel de calidad: 200
• Agency: USP/NF; meets USP testing specifications
• formulario: powder
• potencia: ≥750 μg per mg
• solubilidad: H₂O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)
• espectro de actividad antibiótica: Gram-negative bacteria; Gram-positive bacteria; mycobacteria; mycoplasma
• aplicaciones: pharmaceutical (small molecule)
• Modo de acción: protein synthesis | interferes
• cadena SMILES: OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• Clave InChI: OOYGSFOGFJDDHP-KMCOLRRFSA-N

Descripción

Descripción general

Kanamycin Sulfate is a broad spectrum aminoglycoside-antibiotic purified from the bacterium Streptomyces kanamyceticus. Kanamycin Sulfate is a selection agent for cells in culture media and acts by binding to the 30S subunit of the bacterial ribosome which inhibits protein synthesis in susceptible bacteria.

Aplicación

Kanamycin sulfate is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL. Kanamycin sulfate from Streptomyces kanamyceticus has been used: in the research of cell type-specific expression of genes

Acciones bioquímicas o fisiológicas

Modo de acción: El producto actúa uniéndose a la subunidad ribosómica 70S, inhibiendo la translocación y provocando una codificación errónea.

Modo de resistencia: las enzimas modificadoras de los aminoglucósidos (como la acetiltransferasa, la fosfotransferasa y la nucleotidiltransferasa) pueden alterar este antibiótico y evitar su interacción con los ribosomas.

Espectro antimicrobiano: El sulfato de canamicina es eficaz contra las bacterias gramnegativas y grampositivas, y contra los micoplasmas.

Características y beneficios

• High quality antibiotic suitable for mulitple research applications
• meets USP testing specifications

Nota de preparación

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Almacenamiento y estabilidad

Tightly closed. Dry. Keep in a well -ventilated place. Keep locked up or in an area accessible

Otras notas

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Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H360D
• Consejos de prudencia: P201 - P308 + P313
• Clasificaciones de peligro: Repr. 1B
• Código de clase de almacenamiento: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 2
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)