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93595

Sigma-Aldrich

Azul de tripano solution

0.4%, for microscopy

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About This Item

Fórmula empírica (notación de Hill):
C34H24N6Na4O14S4
Número de CAS:
72-57-1
Peso molecular:
960.81
Beilstein:
4360496
Número MDL:
MFCD00003969
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
329770254
NACRES:
MA.02

grado

for microscopy

Nivel de calidad

200

formulario

liquid

caducidad

limited shelf life, expiry date on the label

concentración

0.4%

color

blue to very dark blue

densidad

1.007 g/mL at 20 °C

εmax

1.4 at 603 nm in methanol

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

Clave InChI

GLNADSQYFUSGOU-UHFFFAOYSA-J

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Descripción general

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Aplicación

Trypan blue applications include:
  • it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
  • employed as a collagen stain in a Van Gieson procedure
  • To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
  • used as a fluorescent tracer of cell populations in embryology
  • used as a tumor promoter modulating permeability of lysosomal membranes
Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Acciones bioquímicas o fisiológicas

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Forma física

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H350

Clasificaciones de peligro

Carc. 1B

Código de clase de almacenamiento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
BCCL2819
BCCL5345
BCCK8639
BCCK2372
BCCJ9155

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

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