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Merck

H9394

Sigma-Aldrich

Sal equilibrada de Hanks solution

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

HBSS

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

esterilidad

sterile-filtered

formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

Condiciones de envío

ambient

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Descripción general

Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.

Aplicación

Hanks′ Balanced Salt solution has been used:
  • as a component of the culture medium for culturing of mouse cortical slices
  • as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)
  • for in situ perfusions of mice liver

Acciones bioquímicas o fisiológicas

Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.

suplemento

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Protocolos

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

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