302643

Trypan Blue

Dye content 60 %, ≥80% (HPLC)

• Sinónimos: Direct blue 14
• Fórmula lineal: C₃₄H₂₄N₆O₁₄S₄Na₄
• Número de CAS: 72-57-1
• Peso molecular: 960.81
• Número de índice de color: 23850
• Beilstein: 4360496
• Número CE: 200-786-7
• Número MDL: MFCD00003969
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24858268
• NACRES: NA.47

Propiedades

• Nivel de calidad: 200
• Ensayo: ≥80% (HPLC)
• Formulario: powder
• composición: Dye content, 60%
• técnicas: microbe id | staining: suitable
• color: dark green to brown, or Brown-Black
• mp: >300 °C (lit.)
• solubilidad: H₂O: 1 mg/mL
• λmáx.: 607 nm
• ε (coeficiente de extinción): ≥60000 at 603-607 nm in methanol at 0.01 g/L; 60000 at 603-607 nm in methanol
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• Clave InChI: GLNADSQYFUSGOU-UHFFFAOYSA-J
• Información sobre el gen: human ... PTPN1(5770) , PTPRF(5792)

Descripción

Descripción general

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.

Aplicación

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Acciones bioquímicas o fisiológicas

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:

• to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
• to stain plant tissue in order to visualize cell death.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H350
• Consejos de prudencia: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Carc. 1B
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges