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Merck

252549

Sigma-Aldrich

Formaldehído solution

Formaldehyde solution

ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

Sinónimos:

Formalina

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About This Item

Fórmula lineal:
HCHO
Número de CAS:
Peso molecular:
30.03
Beilstein:
1209228
Número MDL:
Código UNSPSC:
12352114
ID de la sustancia en PubChem:
NACRES:
NA.21
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grado

ACS reagent

Nivel de calidad

densidad de vapor

1.03 (vs air)

presión de vapor

52 mmHg ( 37 °C)
52 mmHg ( 37 °C)

Formulario

liquid

temp. de autoignición

572 °F

contiene

10-15% Methanol as stabilizer (to prevent polymerization)

concentración

36.5-38.0%
37 wt. % in H2O

impurezas

≤0.006 meq/g Titr. acid

residuo de ign.

≤0.005%

color

APHA: ≤10

índice de refracción

n20/D 1.3765

solubilidad

water: soluble

densidad

1.09 g/mL at 25 °C (lit.)

trazas de anión

chloride (Cl-): ≤5 ppm
sulfate (SO42-): ≤0.002%

trazas de catión

Fe: ≤5 ppm
heavy metals: ≤5 ppm (by ICP-OES)

idoneidad

suitable for manufacturing use (Laboratory chemicals)

cadena SMILES

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

Clave InChI

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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Descripción general

Formaldehyde (formalin) is produced by oxidation of methanol. It is made of 37% formaldehyde and impurities such as methanol, small amounts of formic acid, aldehydes and ketones. It is used as a denaturant in formaldehyde-agarose gel electrophoresis of RNA.[1][2]

Aplicación

Formaldehyde solution has been used as a fixing agent to fix cells during immunofluorescence imaging and for cross-linking cells during chromatin immunoprecipitation (ChIP) assay.[3]
Formaldehyde solution has been used for cross-linking/fixing of cells in ChIP (chromatin immunoprecipitation) assay.[4][5] It has been used for fixing of cells for imaging.[6][7]

Forma física

This product is a solution of approximately 37% by weight of formaldehyde gas in water.

también adquirido normalmente con este producto

Referencia del producto
Descripción
Precios

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

Órganos de actuación

Eyes,Central nervous system, Respiratory system

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

132.8 °F - closed cup

Punto de inflamabilidad (°C)

56 °C - closed cup


Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

EU REACH Annex XVII (Restriction List)


Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

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Visite la Librería de documentos

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SIRT6 protects against endothelial dysfunction and atherosclerosis in mice.
Xu S et al.
Aging (Albany. NY.), 8, 1064-1064 (2016)
Intracellular protein and nucleic acid measured in eight cell types using deep-ultraviolet mass mapping.
Cheung MC et al.
Cytometry, 83, 540-540 (2013)
Farrell RE
RNA Methodologies: A Laboratory Guide for Isolation and Characterization (2010)

Preguntas

1–10 de 16 Preguntas  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 respuesta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 respuesta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Can you store formaldehyde in plastic?

    1 respuesta
    1. This product is packaged in glass bottles. Generally, formaldehyde solution can be used in plastic containers. However, it is advised that the unused material be preserved in its original packaging.

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  4. What type of packaging for different packages?

    1 respuesta
    1. This product is packaged in amber glass bottles with polypropylene caps with HDPE liners. The bottle sizes are as follows:
      252549-25 ML in 50 ml bottles
      252549-100 ML in 100 ml bottles
      252549-500ML in 500 ml bottles
      252549-1L in 1L bottles
      252549- 4L in 4L bottles

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  5. Can formaldehyde solution 37% be diluted to 10% formalin for fixing cells for staining? Do you dilute it with around 2.7 part water? Thank you!

    1 respuesta
    1. How much water should be added to 100mL of a 37% solution to reduce its strength to a 10% solution?
      Use C1 x V1= C2 x V2
      37% x 100ml = 10% x V2
      Therefore V2 = (37% x 100ml) / 10%
      = 370 mL

      Subtract the total original volume from the new volume.
      370 mL - 100 mL = 270 mL
      So to reduce 100mL of 37% to 10% solution, add 270 mL of water to 100 mL of Formaldehyde solution - 252549-25ML.

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  6. Which formaldehyde solution is recommended for use with the HT102A Reticulum kit?

    1 respuesta
    1. When a silver stain procedure recommends a 10% formalin solution, it is referring to a 10% aqueous formalin solution, which is prepared by diluting 1 part of 37% to 40% formaldehyde with 9 parts deionized water. For histology, it is advisable to use ACS reagents when the product meets ACS Specifications. Suitable products for this purpose would be product numbers 252549 or FX0410. If a 37% to 40% formaldehyde solution is available, it should work; however, it's important to avoid using old bottles of this solution that have been in the lab for several years, as impurities can form over time. Solutions used in histological procedures should typically be replaced every 2 years to ensure effectiveness.

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  7. Is the methanol content in the CoA expressed as volume percent or weight percent?

    1 respuesta
    1. The methanol content is expressed as weight percent.

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  8. Why is Product No. 252549, Formaldehyde solution, stabilized with methanol?

    1 respuesta
    1. The methanol serves to slow the formation of paraformaldehyde.

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  9. Do you offer 100% formaldehyde instead of solutions like Product No. 252549?

    1 respuesta
    1. Formaldehyde in its pure form is a gas. The maximum concentration of formaldehyde in aqueous solution is about 37% by weight.  This is equivalent to an approximately 13.3 M solution.

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  10. What is a common application for Product No. 252549, Formaldehyde solution?

    1 respuesta
    1. Formaldehyde can be used in multiple applications in different fields.  One common application is that this product is a common building block for the synthesis of more complex compounds and materials.

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1–10 de 16 Preguntas  

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