8.51025

5-Carboxyfluorescein

Novabiochem^®

• Sinónimos: 5-Carboxyfluorescein, 5-FAM
• Fórmula empírica (notación de Hill): C₂₁H₁₂O₇
• Número de CAS: 76823-03-5
• Peso molecular: 376.32
• Número MDL: MFCD00036874
• Código UNSPSC: 12352005
• NACRES: NA.22

Propiedades

• Nivel de calidad: 200
• Línea del producto: Novabiochem^®
• Análisis: ≥98% (TLC); ≥99.0% (HPLC)
• formulario: powder
• idoneidad de la reacción: reagent type: fluorescent-labelling reagent
• fabricante / nombre comercial: Novabiochem^®
• aplicaciones: peptide synthesis
• temp. de almacenamiento: 2-8°C
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• Clave InChI: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Descripción

Descripción general

This high purity fluorescent dye (λ_ex494 nm, λ_em 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment ^[1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Aplicación

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

Ligadura / enlace

Replaces: 01-63-0112

Nota de análisis

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Información legal

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable