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Sigma-Aldrich

Fluorescein 5(6)-isothiocyanate

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

Sinónimos:

FITC, Fluorescein isothiocyanate

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About This Item

Fórmula empírica (notación de Hill):
C21H11NO5S
Número de CAS:
27072-45-3
Peso molecular:
389.38
Beilstein:
1407295
Número CE:
248-207-7
Número MDL:
MFCD00005063
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
329757273
NACRES:
NA.32

Línea del producto

BioReagent

Análisis

≥90% (HPLC)

fluorescencia

λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

idoneidad

suitable for fluorescence

temp. de almacenamiento

−20°C

cadena SMILES

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

Clave InChI

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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Descripción general

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

Aplicación

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Envase

Bottomless glass bottle. Contents are inside inserted fused cone.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

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