F7250

Fluorescein isothiocyanate isomer I

≥90% purity (HPLC), powder

• Sinónimos: FITC, Fluorescein 5-isothiocyanate
• Fórmula empírica (notación de Hill): C₂₁H₁₁NO₅S
• Número de CAS: 3326-32-7
• Peso molecular: 389.38
• Beilstein: 1407295
• Número CE: 222-042-0
• Número MDL: MFCD00005063
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24894933
• NACRES: NA.47

Propiedades

• product name: Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
• Nivel de calidad: 200
• Análisis: ≥90% (HPLC)
• formulario: powder
• color: orange to dark orange
• mp: >360 °C (lit.)
• solubilidad: acetone: 1 mg/mL
• fluorescencia: λ_ex 492 nm; λ_em 518 nm (green)
• idoneidad: suitable for protein labeling
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: 2-8°C
• cadena SMILES: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• Clave InChI: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Descripción

Descripción general

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Aplicación

Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.

El reactivo para el marcado con FITC de proteínas; microsecuenciado de proteínas y péptidos (HPLC)

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317,H334
• Consejos de prudencia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Faceshields, Gloves