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TOX1

Sigma-Aldrich

In Vitro Toxicology Assay Kit, MTT based

Sinónimos:

mitochondrial activity assay

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.84

uso

1 kit sufficient for 1,000 tests

Nivel de calidad

200

envase

pkg of 1 kit

condiciones de almacenamiento

dry at room temperature

λmáx.

570 nm

aplicaciones

cell analysis
detection

método de detección

colorimetric

temp. de almacenamiento

2-8°C

Descripción general

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Aplicación

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Acciones bioquímicas o fisiológicas

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

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Palabra de señalización

Danger

Clasificaciones de peligro

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

Órganos de actuación

Central nervous system, Respiratory system

Código de clase de almacenamiento

3 - Flammable liquids

Punto de inflamabilidad (°F)

53.6 °F

Punto de inflamabilidad (°C)

12 °C

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Certificados de análisis (COA)

Lot/Batch Number
SLCR1314
SLCR1315
SLCL3638
SLCJ1228
SLCG9357

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Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
Baumgart J, et al.
Biomaterials, 33(7), 2345-2350 (2012)
Dalia Chávez-García et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 108(6), 2396-2406 (2020-02-06)
Luminescent lanthanide downconversion nanoparticles (DCNPs) provide a combination of high luminescence intensity, sharp emission peaks with narrow bandwidth and a large Stokes' shift, leading to high-performance biomedical applications mainly for imaging. The purpose of this study is to present a
Human renal organic anion transporter 1-dependent uptake and toxicity of mercuric-thiol conjugates in Madin-Darby canine kidney cells
Aslamkhan AG, et al.
Molecular Pharmacology, 63(3), 590-596 (2003)
J Carmichael et al.
Cancer research, 47(4), 936-942 (1987-02-15)
Drug sensitivity assays were performed using a variation of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay on V79, CHO-AuxB1, CHRC5, NCI-H460, and NCI-H249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with
F Denizot et al.
Journal of immunological methods, 89(2), 271-277 (1986-05-22)
A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells
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