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M2128

Azul de tiazolil Bromuro de tetrazolio

98%, powder

Sinónimos:

Bromuro de 3-(4,5-dimetil-2-tiazolil)-2,5-difenil-2H-tetrazolio, Bromuro de metiltiazolildifenil-tetrazolio, MTT

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Fórmula empírica (notación de Hill):
C18H16BrN5S
Número CAS:
298-93-1
Peso molecular:
414.32
NACRES:
NA.47
PubChem Substance ID:
24896603
UNSPSC Code:
12352111
EC Number:
206-069-5
MDL number:
MFCD00011964
Beilstein/REAXYS Number:
4081397
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Nombre del producto

Azul de tiazolil Bromuro de tetrazolio, 98%

Quality Segment

200

assay

98%

form

powder

technique(s)

titration: suitable

color

dark yellow

mp

195 °C (dec.) (lit.)

solubility

2-methoxyethanol: soluble 20 mg/mL, ethanol: soluble 20 mg/mL, H2O: 5 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

Application

El azul de tiazolil bromuro de tetrazolio se ha utilizado para los siguientes análisis:
  • Ensayos de proliferación .
  • TNF (factor de necrosis tumoral) bioanálisis.
El azul de tiazolil Azul tetrazolio (MTT) puede utilizarse para medir la proliferación celular. El MTT produce una disolución amarillenta que vira a formazano de MTT azul violeta insoluble en agua por acción de las deshidrogenasas mitocondriales de las células vivas. Los cristales azules se solubilizan con isopropanol acidificado y la intensidad se mide colorimétricamente a 570 nm.
El azul de tiazolil Azul tetrazolio (MTT) puede utilizarse para medir la proliferación celular. El MTT produce una disolución amarillenta que vira a formazano de MTT azul violeta insoluble en agua por acción de las deshidrogenasas mitocondriales de las células vivas. Los cristales azules se solubilizan con isopropanol acidificado y la intensidad se mide colorimétricamente a 570 nm. El MTT se ha utilizado como un reactivo histoquímico o citoquímico y para la detección del NAD. Los sistemas enzimáticos ligados a ADP de los tejidos no pueden detectarse con MTT, debido a la unión del catión por la trampa de cianuro utilizada. El MTT se reduce rápidamente al formazano, que forma complejos con el níquel, el cobre y el cobalto; los quelatos de cobalto se han utilizado en los sistemas oxidativos.

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Este artículo
475989M5655T4375
Azul de tiazolil Bromuro de tetrazolio 98%

Sigma-Aldrich

M2128

Azul de tiazolil Bromuro de tetrazolio

MTT Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

Sigma-Aldrich

475989

MTT

Azul de tiazolil Bromuro de tetrazolio powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

Sigma-Aldrich

M5655

Azul de tiazolil Bromuro de tetrazolio

Nitroazul de tetrazolio used in colorimetric determination of reducing compounds

Sigma-Aldrich

T4375

Nitroazul de tetrazolio

description

98%

description

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

description

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

description

used in colorimetric determination of reducing compounds

technique(s)

titration: suitable

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

color

dark yellow

color

yellow

color

-

color

-

form

powder

form

solid

form

powder

form

powder

solubility

ethanol: soluble 20 mg/mL, H2O: 5 mg/mL, 2-methoxyethanol: soluble 20 mg/mL

solubility

PBS: 2 mg/mL

solubility

H2O: 5 mg/mL

solubility

methanol: 50 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Questions

1–10 of 10 Questions  

  1. I used 0.05g in 10ml then syringe it and add 40ml. My question is how to calculate the percentage of viable cells? I used DMSO after MTT solution

    1 answer

    1. MTT is typically used for the semi-quantitative colorimetric measurement of cell viability. This analysis is performed in a multi-well format which screens for the viability of any given compound at any given concentration. The percentage of viable cells cannot be determined using this method. After adding a stop reagent, in this case DMSO, the formazan crystals will be dissolved, making manual counting a challenge, at best. When used in a multi-well plate format, cells are incubated, a stop solution is added, and the plate is read spectrophotometrically at 570 nM. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/305/017/m2128pis.pdf

      For additional information, see the link below to the MTT kit protocol for product TOX1, a cytotoxicity kit that utilized MTT:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/177/tox1bul.pdf

      To measure cell viability as a percentage of an entire population, microscopic methods may be of interest. Reagents such as Trypan Blue allow for the clear identification of viable and non-viable cells. To review more information regarding this method, please see the link below:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/cell-quantification

      Helpful?

  2. What is the recommendation volume of 5 mg/ml of thiazolyl blue tetrazolium blue (in uL) per well to use for MTT assay? 

    1 answer

    1. Instructions for use are not provided for this product. However, there is an in vitro toxicology assay kit MTT based available. The catalog number is TOX1-1KT. In the kit, 15 mg of solid MTT is supplied. This is resuspended with 3 ml of media or a balanced salt solution without phenol red or serum. The link shared below will list the complete procedure

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/177/tox1bul.pdf

      Helpful?

  3. How can I store solutions of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 answer

    1. Reconstituted MTT solution is stable for at least 6 months when stored frozen (-20 °C). Storage of reconstituted MTT solution at 2-8°C for more than 2 weeks may cause decomposition and yield erroneous results.

      Helpful?

  4. How can Thiazolyl Blue Tetrazolium Bromide be used for staining dehydrogenase in tissue sections?

    1 answer

    1. Products M2128 or M5655, Thiazolyl Blue Tetrazolium Bromide can be used for staining dehydrogenase in tissue sections. See the method below. Reagent PreparationMTT (2 mg/ml distilled water) 2.5 ml0.2 M Tris buffer, pH 7.4 2.5 ml0.5 M cobalt chloride 0.5 ml0.05 M magnesium chloride 1.0 mlDistilled water 2.5 mlThe pH of this solution is adjusted to 7.0 if necessary. Aliquot and store at -20°C until required. Sample PreparationCut cytostat sections at 5 to 7 microns. Do not fix. Method:1. Incubate sections in appropriate incubation solution at 37°C for 30-60 minutes.2. Transfer sections to 15% formol saline, 15 minutes.3. Wash in distilled water4. Counterstain in 2% methyl green if required.5. Wash in distilled water.6. Mount in glycerin Jelly. ResultsEnzyme: Black formazan deposits with MTT Source:  Bancroft and Gamble, Theory and Practice of Histological Techniques, Fifth Edition. Churchill Livingstone, London, 2002, page 610.

      Helpful?

  5. What can be used to make a solution of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 answer

    1. We test the solubility of MTT in water at a concentration of 5 mg/mL.  For the MTT viability assay, we dissolve Product No. M5655 at a concentration of 5 mg/ml in RPMI-1640 without phenol red. This medium is available as a powder (Product No.R8755) or liquid (Product No. R7509). MTT can be solubilized in any culture media or balanced salt solution that does not contain phenol red as a pH indicator.

      Helpful?

  6. Is the MTT assay quantitative?

    1 answer

    1. The MTT asay is a method to assess cell viability.  This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.

      Helpful?

  7. What can be used to solubilize MTT formazan (the product of the Thiazolyl Blue Tetrazolium Bromide - MTT reaction)?

    1 answer

    1. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring of the MTT, yielding purple MTT formazan crystals. These crystals are not soluble in aqueous solutions; they may be dissolved in acidified isopropanol (0.1 N HCl in anhydrous isopropanol). If this does not lyse the cells, Triton X-100 can be added to the acidified isopropanol. A typical working concentration is 10% Triton X-100 (i.e., 1 volume of 100% Triton X-100 + 9 volumes of acidified isopropanol).

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Why is a reference wavelength used for data analysis of Thiazolyl Blue Tetrazolium Bromide (MTT)?

    1 answer

    1. The reference wavelength (630-690 nm) is used to adjust for the optical variation in the wells of the plate.  It also corrects for dust and fingerprints that can be present.  The assay is read at 570 nm.  The reference wavelength needs to be far enough away from the assay wavelength so as not to affect those values.

      Helpful?

  10. What type of multi-well plate should be used for the Thiazolyl Blue Tetrazolium Bromide (MTT) assay?

    1 answer

    1. It is recommended that flat bottom multi-well plates be used for the MTT assay. The use of tissue culture treated plates is only important if necessary for good cell growth.  If cells are grown in larger plates, the supernatant after cell lysis can be transferred to a 96 well plate for reading.

      Helpful?

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