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CGD1

Sigma-Aldrich

Cell Growth Determination Kit, MTT based

Sinónimos:

Mitochondrial activity assay

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

envase

pkg of 1 kit

Nivel de calidad

200

técnicas

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

aplicaciones

cell analysis
detection

método de detección

colorimetric

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Aplicación

Cell Growth Determination Kit, MTT based has been used:
  • to measure the cell viability of preosteoblastic cells
  • in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
  • to determine the cell viability of primary cervical epithelial cells

Envase

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

Producto relacionado

Referencia del producto
Descripción
Precios

06693

Timestrip Plus™ -20 °C

Palabra de señalización

Danger

Frases de peligro

H225,H319,H336,H341

Clasificaciones de peligro

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

Órganos de actuación

Central nervous system

Código de clase de almacenamiento

3 - Flammable liquids

Punto de inflamabilidad (°F)

53.6 °F - closed cup

Punto de inflamabilidad (°C)

12 °C - closed cup

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Certificados de análisis (COA)

Lot/Batch Number
SLCP8351
SLCM0387
SLCK0987
SLCF3922
SLCC1767

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