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TOX8

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Resazurin based

Sinónimos:

Fluorescent resazurin toxicology assay

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About This Item

EC Number:
263-718-5
UNSPSC Code:
12352207
NACRES:
NA.84

form

liquid

usage

1 kit sufficient for 2,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

protein quantification: suitable

application(s)

cell analysis
detection

detection method

colorimetric
fluorometric

storage temp.

2-8°C

General description

In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible

Application

In Vitro Toxicology Assay Kit, Resazurin based has been used:
  • in cell viability assay of human primary fibroblasts
  • to determine the cell proliferation rate in murine pre-osteoblasts
  • to determine the metabolic activity of microfluidic and monolayer cultures

Biochem/physiol Actions

Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

Related product

Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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A rapid biochemical test for measuring chemical toxicity.
D Liu
Bulletin of environmental contamination and toxicology, 26(2), 145-149 (1981-02-01)
Comparison of several microbiological toxicity screening tests.
Dutka, B.J., et al.
Water Research, 17, 1363-1367 (1983)
Monika Marędziak et al.
Stem cells international, 2016, 2152435-2152435 (2016-03-05)
Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic
A comparative study of methods for assessing the toxicity to bacteria of single chemicals and mixtures.
King, E.F. et al.
Toxicity Screening Procedures Using Bacterial Systems, 175-194 (1984)
U J Strotmann et al.
Ecotoxicology and environmental safety, 25(1), 79-89 (1993-02-01)
The dehydrogenase assay with resazurin as an oxidoreduction dye was used for monitoring the performance of six laboratory-scale sewage treatment plants. The dehydrogenase activity measured proved to be a reliable monitoring parameter, although it did not always positively correlate with
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